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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PRKCQ

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PRKCQ PRKCT
Sequence Length (AA) Molecular Weight (Da)
706 81865
Protein Name
Protein kinase C theta type
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSPFLRIGLS NFDCGSCQSC QGEAVNPYCA VLVKEYVESE NGQMYIQKKP
60 70 80 90 100
TMYPPWDSTF DAHINKGRVM QIIVKGKNVD LISETTVELY SLAERCRKNN
110 120 130 140 150
GKTEIWLELK PQGRMLMNAR YFLEMSDTKD MNEFETEGFF ALHQRRGAIK
160 170 180 190 200
QAKVHHVKCH EFTATFFPQP TFCSVCHEFV WGLNKQGYQC RQCNAAIHKK
210 220 230 240 250
CIDKVIAKCT GSAINSRETM FHKERFKIDM PHRFKVYNYK SPTFCEHCGT
260 270 280 290 300
LLWGLARQGL KCDACGMNVH HRCQTKVANL CGINQKLMAE ALAMIESTQQ
310 320 330 340 350
ARCLRDTEQI FREGPVEIGL PCSIKNEARP PCLPTPGKRE PQGISWESPL
360 370 380 390 400
DEVDKMCHLP EPELNKERPS LQIKLKIEDF ILHKMLGKGS FGKVFLAEFK
410 420 430 440 450
KTNQFFAIKA LKKDVVLMDD DVECTMVEKR VLSLAWEHPF LTHMFCTFQT
460 470 480 490 500
KENLFFVMEY LNGGDLMYHI QSCHKFDLSR ATFYAAEIIL GLQFLHSKGI
510 520 530 540 550
VYRDLKLDNI LLDKDGHIKI ADFGMCKENM LGDAKTNTFC GTPDYIAPEI
560 570 580 590 600
LLGQKYNHSV DWWSFGVLLY EMLIGQSPFH GQDEEELFHS IRMDNPFYPR
610 620 630 640 650
WLEKEAKDLL VKLFVREPEK RLGVRGDIRQ HPLFREINWE ELERKEIDPP
660 670 680 690 700
FRPKVKSPFD CSNFDKEFLN EKPRLSFADR ALINSMDQNM FRNFSFMNPG
706
MERLIS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2825 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRKCQ
Peptide Modified Sequence
SPFDC[+57.0]SNFDK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
661
Peptide - Site of Modification
5
Peptide Start
657
Peptide End
666
CPTAC ID
CPTAC-2825
Peptide Molecular Mass
1,215.4866
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 7.6 3.4 2.8 7.3 3.5 6.1 10.5 4.9 6.7 15 15 15
y6 (1+) 7.2 3.6 3 6 3.5 6.7 9.4 5 7.3 15 15 15
b3 (1+) 29.8 5.1 5.9 61.6 4.9 8.5 68.4 7.1 10.3 15 15 15
sum 8.1 3.1 2.8 9.4 3 6.2 12.4 4.3 6.8 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Comments

Assay Details for non-CPTAC-2890 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRKCQ
Peptide Modified Sequence
SPTFC[+57.0]EHC[+57.0]GTLLWGLAR
Modification Type
Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification
246, 249
Peptide - Site of Modification
5, 8
Peptide Start
241
Peptide End
257
CPTAC ID
non-CPTAC-2890
Peptide Molecular Mass
2,003.9346
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 8.1 4.3 4.4 8.7 4.7 4.7 11.9 6.4 6.4 15 15 15
y4 (1+) 7.1 3.5 4.5 7.9 4.2 5.3 10.6 5.5 7 15 15 15
y6 (1+) 7.6 5.2 4.2 8.3 6.1 4.8 11.3 8 6.4 15 15 15
sum 7.2 4 4.1 7.4 4.5 4.5 10.3 6 6.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments