DCLK3, CPTAC-2833 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
DCLK3	DCAMKL3, DCDC3C, KIAA1765

Sequence Length (AA)	Molecular Weight (Da)
648	73814

Protein Name
Serine/threonine-protein kinase DCLK3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGKEPLTLKS	IQVAVEELYP	NKARALTLAQ	HSRAPSPRLR	SRLFSKALKG
60	70	80	90	100
DHRCGETETP	KSCSEVAGCK	AAMRHQGKIP	EELSLDDRAR	TQKKWGRGKW
110	120	130	140	150
EPEPSSKPPR	EATLEERHAR	GEKHLGVEIE	KTSGEIIRCE	KCKRERELQQ
160	170	180	190	200
SLERERLSLG	TSELDMGKGP	MYDVEKLVRT	RSCRRSPEAN	PASGEEGWKG
210	220	230	240	250
DSHRSSPRNP	TQELRRPSKS	MDKKEDRGPE	DQESHAQGAA	KAKKDLVEVL
260	270	280	290	300
PVTEEGLREV	KKDTRPMSRS	KHGGWLLREH	QAGFEKLRRT	RGEEKEAEKE
310	320	330	340	350
KKPCMSGGRR	MTLRDDQPAK	LEKEPKTRPE	ENKPERPSGR	KPRPMGIIAA
360	370	380	390	400
NVEKHYETGR	VIGDGNFAVV	KECRHRETRQ	AYAMKIIDKS	RLKGKEDMVD
410	420	430	440	450
SEILIIQSLS	HPNIVKLHEV	YETDMEIYLI	LEYVQGGDLF	DAIIESVKFP
460	470	480	490	500
EPDAALMIMD	LCKALVHMHD	KSIVHRDLKP	ENLLVQRNED	KSTTLKLADF
510	520	530	540	550
GLAKHVVRPI	FTVCGTPTYV	APEILSEKGY	GLEVDMWAAG	VILYILLCGF
560	570	580	590	600
PPFRSPERDQ	DELFNIIQLG	HFEFLPPYWD	NISDAAKDLV	SRLLVVDPKK
610	620	630	640	648
RYTAHQVLQH	PWIETAGKTN	TVKRQKQVSP	SSEGHFRSQH	KRVVEQVS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2833 Collapse assay details

Data source: Panorama

Official Gene Symbol: DCLK3
Peptide Sequence: QVSPSSEGHFR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 627
Peptide End: 637

CPTAC ID: CPTAC-2833
Peptide Molecular Mass: 1,229.5789
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive™
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC™ 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	17.2	3.8	5.1	15.6	5.5	5.4	23.2	6.7	7.4	15	15	15
y5 (1+)	27.9	4	5	37.6	7.1	7.3	46.8	8.1	8.8	15	15	15
y4 (1+)	11	3.4	3.3	24	4.2	4.3	26.4	5.4	5.4	15	15	15
sum	8.4	2.8	2.9	9.3	3.4	3.3	12.5	4.4	4.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2832 Collapse assay details

Data source: Panorama

Official Gene Symbol: DCLK3
Peptide Sequence: VIGDGNFAVVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 361
Peptide End: 371

CPTAC ID: CPTAC-2832
Peptide Molecular Mass: 1,117.6132
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend