SRMS, CPTAC-2834 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SRMS	C20orf148

Sequence Length (AA)	Molecular Weight (Da)
488	54507

Protein Name
Tyrosine-protein kinase Srms

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEPFLRRRLA	FLSFFWDKIW	PAGGEPDHGT	PGSLDPNTDP	VPTLPAEPCS
60	70	80	90	100
PFPQLFLALY	DFTARCGGEL	SVRRGDRLCA	LEEGGGYIFA	RRLSGQPSAG
110	120	130	140	150
LVPITHVAKA	SPETLSDQPW	YFSGVSRTQA	QQLLLSPPNE	PGAFLIRPSE
160	170	180	190	200
SSLGGYSLSV	RAQAKVCHYR	VSMAADGSLY	LQKGRLFPGL	EELLTYYKAN
210	220	230	240	250
WKLIQNPLLQ	PCMPQKAPRQ	DVWERPHSEF	ALGRKLGEGY	FGEVWEGLWL
260	270	280	290	300
GSLPVAIKVI	KSANMKLTDL	AKEIQTLKGL	RHERLIRLHA	VCSGGEPVYI
310	320	330	340	350
VTELMRKGNL	QAFLGTPEGR	ALRLPPLLGF	ACQVAEGMSY	LEEQRVVHRD
360	370	380	390	400
LAARNVLVDD	GLACKVADFG	LARLLKDDIY	SPSSSSKIPV	KWTAPEAANY
410	420	430	440	450
RVFSQKSDVW	SFGVLLHEVF	TYGQCPYEGM	TNHETLQQIM	RGYRLPRPAA
460	470	480	488
CPAEVYVLML	ECWRSSPEER	PSFATLREKL	HAIHRCHP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2951 Collapse assay details

Data source: Panorama

Official Gene Symbol: SRMS
Peptide Modified Sequence: NVLVDDGLAC[+57.0]K
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 365
Peptide - Site of Modification: 10
Peptide Start: 355
Peptide End: 365

CPTAC ID: CPTAC-2951
Peptide Molecular Mass: 1,202.5965
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	6.8	8.1	6.2	10.2	8.5	5.7	12.3	11.7	8.4	15	15	15
y7 (1+)	7.9	10	4.8	7.5	8.8	7	10.9	13.3	8.5	15	15	15
y5 (1+)	8.8	9.3	7.2	11.9	8	8.7	14.8	12.3	11.3	15	15	15
sum	7	8.6	5	8.4	8	5.8	10.9	11.7	7.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2834 Collapse assay details

Data source: Panorama

Official Gene Symbol: SRMS
Peptide Sequence: SSPEERPSFATLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 465
Peptide End: 477

CPTAC ID: CPTAC-2834
Peptide Molecular Mass: 1,475.7369
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend