NLK, CPTAC-2835 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NLK	LAK1

Sequence Length (AA)	Molecular Weight (Da)
527	58283

Protein Name
Serine/threonine-protein kinase NLK

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSLCGARANA	KMMAAYNGGT	SAAAAGHHHH	HHHHLPHLPP	PHLHHHHHPQ
60	70	80	90	100
HHLHPGSAAA	VHPVQQHTSS	AAAAAAAAAA	AAAMLNPGQQ	QPYFPSPAPG
110	120	130	140	150
QAPGPAAAAP	AQVQAAAAAT	VKAHHHQHSH	HPQQQLDIEP	DRPIGYGAFG
160	170	180	190	200
VVWSVTDPRD	GKRVALKKMP	NVFQNLVSCK	RVFRELKMLC	FFKHDNVLSA
210	220	230	240	250
LDILQPPHID	YFEEIYVVTE	LMQSDLHKII	VSPQPLSSDH	VKVFLYQILR
260	270	280	290	300
GLKYLHSAGI	LHRDIKPGNL	LVNSNCVLKI	CDFGLARVEE	LDESRHMTQE
310	320	330	340	350
VVTQYYRAPE	ILMGSRHYSN	AIDIWSVGCI	FAELLGRRIL	FQAQSPIQQL
360	370	380	390	400
DLITDLLGTP	SLEAMRTACE	GAKAHILRGP	HKQPSLPVLY	TLSSQATHEA
410	420	430	440	450
VHLLCRMLVF	DPSKRISAKD	ALAHPYLDEG	RLRYHTCMCK	CCFSTSTGRV
460	470	480	490	500
YTSDFEPVTN	PKFDDTFEKN	LSSVRQVKEI	IHQFILEQQK	GNRVPLCINP
510	520	527
QSAAFKSFIS	STVAQPSEMP	PSPLVWE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2964 Collapse assay details

Data source: Panorama

Official Gene Symbol: NLK
Peptide Sequence: EIIHQFILEQQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 479
Peptide End: 490

CPTAC ID: CPTAC-2964
Peptide Molecular Mass: 1,524.8300
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	7.1	4.7	7.8	7.1	4.9	10.9	10	6.8	13.4	15	15	15
b6 (1+)	17.2	5.6	6.4	20.6	8.6	10.1	26.8	10.3	12	15	15	15
y4 (1+)	8.2	5.5	7.8	9.4	6	11.6	12.5	8.1	14	15	15	15
sum	3.9	4.8	7.5	5.3	5.3	10.9	6.6	7.2	13.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2835 Collapse assay details

Data source: Panorama

Official Gene Symbol: NLK
Peptide Sequence: IIVSPQPLSSDHVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 229
Peptide End: 242

CPTAC ID: CPTAC-2835
Peptide Molecular Mass: 1,518.8406
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend