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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
NLK LAK1
Sequence Length (AA) Molecular Weight (Da)
527 58283
Protein Name
Serine/threonine-protein kinase NLK
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSLCGARANA KMMAAYNGGT SAAAAGHHHH HHHHLPHLPP PHLHHHHHPQ
60 70 80 90 100
HHLHPGSAAA VHPVQQHTSS AAAAAAAAAA AAAMLNPGQQ QPYFPSPAPG
110 120 130 140 150
QAPGPAAAAP AQVQAAAAAT VKAHHHQHSH HPQQQLDIEP DRPIGYGAFG
160 170 180 190 200
VVWSVTDPRD GKRVALKKMP NVFQNLVSCK RVFRELKMLC FFKHDNVLSA
210 220 230 240 250
LDILQPPHID YFEEIYVVTE LMQSDLHKII VSPQPLSSDH VKVFLYQILR
260 270 280 290 300
GLKYLHSAGI LHRDIKPGNL LVNSNCVLKI CDFGLARVEE LDESRHMTQE
310 320 330 340 350
VVTQYYRAPE ILMGSRHYSN AIDIWSVGCI FAELLGRRIL FQAQSPIQQL
360 370 380 390 400
DLITDLLGTP SLEAMRTACE GAKAHILRGP HKQPSLPVLY TLSSQATHEA
410 420 430 440 450
VHLLCRMLVF DPSKRISAKD ALAHPYLDEG RLRYHTCMCK CCFSTSTGRV
460 470 480 490 500
YTSDFEPVTN PKFDDTFEKN LSSVRQVKEI IHQFILEQQK GNRVPLCINP
510 520 527
QSAAFKSFIS STVAQPSEMP PSPLVWE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2964 Collapse assay details

Data source: Panorama

Official Gene Symbol
NLK
Peptide Sequence
EIIHQFILEQQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
479
Peptide End
490
CPTAC ID
CPTAC-2964
Peptide Molecular Mass
1,524.8300
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 7.1 4.7 7.8 7.1 4.9 10.9 10 6.8 13.4 15 15 15
b6 (1+) 17.2 5.6 6.4 20.6 8.6 10.1 26.8 10.3 12 15 15 15
y4 (1+) 8.2 5.5 7.8 9.4 6 11.6 12.5 8.1 14 15 15 15
sum 3.9 4.8 7.5 5.3 5.3 10.9 6.6 7.2 13.2 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2835 Collapse assay details

Data source: Panorama

Official Gene Symbol
NLK
Peptide Sequence
IIVSPQPLSSDHVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
229
Peptide End
242
CPTAC ID
CPTAC-2835
Peptide Molecular Mass
1,518.8406
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 24.9 11.1 9.7 39.3 14.2 14.3 46.5 18 17.3 15 15 15
y6 (1+) 8.7 7.8 5.1 8.8 7.2 5.1 12.4 10.6 7.2 15 15 15
b4 (1+) 8.3 8.6 5.9 12.6 7.8 7.7 15.1 11.6 9.7 15 15 15
sum 8.7 7.6 5.5 10.6 7.7 6.1 13.7 10.8 8.2 15 15 15


Additional Resources and Comments