CSNK1A1, CPTAC-2840 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CSNK1A1	N/A

Sequence Length (AA)	Molecular Weight (Da)
337	38915

Protein Name
Casein kinase I isoform alpha

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MASSSGSKAE	FIVGGKYKLV	RKIGSGSFGD	IYLAINITNG	EEVAVKLESQ
60	70	80	90	100
KARHPQLLYE	SKLYKILQGG	VGIPHIRWYG	QEKDYNVLVM	DLLGPSLEDL
110	120	130	140	150
FNFCSRRFTM	KTVLMLADQM	ISRIEYVHTK	NFIHRDIKPD	NFLMGIGRHC
160	170	180	190	200
NKLFLIDFGL	AKKYRDNRTR	QHIPYREDKN	LTGTARYASI	NAHLGIEQSR
210	220	230	240	250
RDDMESLGYV	LMYFNRTSLP	WQGLKAATKK	QKYEKISEKK	MSTPVEVLCK
260	270	280	290	300
GFPAEFAMYL	NYCRGLRFEE	APDYMYLRQL	FRILFRTLNH	QYDYTFDWTM
310	320	330	337
LKQKAAQQAA	SSSGQGQQAQ	TPTGKQTDKT	KSNMKGF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3147 Collapse assay details

Data source: Panorama

Official Gene Symbol: CSNK1A1
Peptide Sequence: ILQGGVGIPHIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 66
Peptide End: 77

CPTAC ID: CPTAC-3147
Peptide Molecular Mass: 1,258.7510
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	6.2	7.2	4.6	10.4	7.3	5	12.1	10.3	6.8	15	15	15
y4 (1+)	4.7	6.3	3.9	8	6	4.5	9.3	8.7	6	15	15	15
y6 (1+)	4.9	6.1	4.2	9.3	6.7	4.8	10.5	9.1	6.4	15	15	15
sum	4.4	6.3	3.9	8.3	6.3	4.5	9.4	8.9	6	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2840 Collapse assay details

Data source: Panorama

Official Gene Symbol: CSNK1A1
Peptide Sequence: YASINAHLGIEQSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 187
Peptide End: 200

CPTAC ID: CPTAC-2840
Peptide Molecular Mass: 1,557.7900
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend