BMPR1B, CPTAC-2846 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
BMPR1B	N/A

Sequence Length (AA)	Molecular Weight (Da)
502	56930

Protein Name
Bone morphogenetic protein receptor type-1B

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLLRSAGKLN	VGTKKEDGES	TAPTPRPKVL	RCKCHHHCPE	DSVNNICSTD
60	70	80	90	100
GYCFTMIEED	DSGLPVVTSG	CLGLEGSDFQ	CRDTPIPHQR	RSIECCTERN
110	120	130	140	150
ECNKDLHPTL	PPLKNRDFVD	GPIHHRALLI	SVTVCSLLLV	LIILFCYFRY
160	170	180	190	200
KRQETRPRYS	IGLEQDETYI	PPGESLRDLI	EQSQSSGSGS	GLPLLVQRTI
210	220	230	240	250
AKQIQMVKQI	GKGRYGEVWM	GKWRGEKVAV	KVFFTTEEAS	WFRETEIYQT
260	270	280	290	300
VLMRHENILG	FIAADIKGTG	SWTQLYLITD	YHENGSLYDY	LKSTTLDAKS
310	320	330	340	350
MLKLAYSSVS	GLCHLHTEIF	STQGKPAIAH	RDLKSKNILV	KKNGTCCIAD
360	370	380	390	400
LGLAVKFISD	TNEVDIPPNT	RVGTKRYMPP	EVLDESLNRN	HFQSYIMADM
410	420	430	440	450
YSFGLILWEV	ARRCVSGGIV	EEYQLPYHDL	VPSDPSYEDM	REIVCIKKLR
460	470	480	490	500
PSFPNRWSSD	ECLRQMGKLM	TECWAHNPAS	RLTALRVKKT	LAKMSESQDI
502
KL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2847 Collapse assay details

Data source: Panorama

Official Gene Symbol: BMPR1B
Peptide Sequence: DLHPTLPPLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 105
Peptide End: 114

CPTAC ID: CPTAC-2847
Peptide Molecular Mass: 1,129.6495
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	4	3.2	3.6	7.2	3.9	2.4	8.2	5	4.3	15	15	15
y4 (1+)	4	2.9	2.9	8.1	3.6	2.6	9	4.6	3.9	15	15	15
y3 (1+)	4.8	3.7	4.2	7.6	4.1	3.1	9	5.5	5.2	15	15	15
sum	4	3.2	3.4	7.6	3.7	2.4	8.6	4.9	4.2	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2846 Collapse assay details

Data source: Panorama

Official Gene Symbol: BMPR1B
Peptide Modified Sequence: NGTC[+57.0]C[+57.0]IADLGLAVK
Modification Type: Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification: 347, 348
Peptide - Site of Modification: 4, 5
Peptide Start: 343
Peptide End: 356

CPTAC ID: CPTAC-2846
Peptide Molecular Mass: 1,490.7221
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend