ACVR1, CPTAC-2848 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ACVR1	ACVRLK2

Sequence Length (AA)	Molecular Weight (Da)
509	57153

Protein Name
Activin receptor type-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVDGVMILPV	LIMIALPSPS	MEDEKPKVNP	KLYMCVCEGL	SCGNEDHCEG
60	70	80	90	100
QQCFSSLSIN	DGFHVYQKGC	FQVYEQGKMT	CKTPPSPGQA	VECCQGDWCN
110	120	130	140	150
RNITAQLPTK	GKSFPGTQNF	HLEVGLIILS	VVFAVCLLAC	LLGVALRKFK
160	170	180	190	200
RRNQERLNPR	DVEYGTIEGL	ITTNVGDSTL	ADLLDHSCTS	GSGSGLPFLV
210	220	230	240	250
QRTVARQITL	LECVGKGRYG	EVWRGSWQGE	NVAVKIFSSR	DEKSWFRETE
260	270	280	290	300
LYNTVMLRHE	NILGFIASDM	TSRHSSTQLW	LITHYHEMGS	LYDYLQLTTL
310	320	330	340	350
DTVSCLRIVL	SIASGLAHLH	IEIFGTQGKP	AIAHRDLKSK	NILVKKNGQC
360	370	380	390	400
CIADLGLAVM	HSQSTNQLDV	GNNPRVGTKR	YMAPEVLDET	IQVDCFDSYK
410	420	430	440	450
RVDIWAFGLV	LWEVARRMVS	NGIVEDYKPP	FYDVVPNDPS	FEDMRKVVCV
460	470	480	490	500
DQQRPNIPNR	WFSDPTLTSL	AKLMKECWYQ	NPSARLTALR	IKKTLTKIDN
509
SLDKLKTDC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2849 Collapse assay details

Data source: Panorama

Official Gene Symbol: ACVR1
Peptide Sequence: NITAQLPTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 102
Peptide End: 110

CPTAC ID: CPTAC-2849
Peptide Molecular Mass: 984.5604
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	14.1	3.8	4.4	15.1	5.4	6.2	20.7	6.6	7.6	15	15	15
y5 (1+)	16.6	6.1	6.7	25.2	8.2	8.6	30.2	10.2	10.9	15	15	15
y6 (1+)	11.4	3.9	6.8	13.4	5.3	6.8	17.6	6.6	9.6	15	15	15
sum	9.7	3.2	4	14.2	4.7	5.3	17.2	5.7	6.6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2848 Collapse assay details

Data source: Panorama

Official Gene Symbol: ACVR1
Peptide Modified Sequence: YMAPEVLDETIQVDC[+57.0]FDSYK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 396
Peptide - Site of Modification: 15
Peptide Start: 381
Peptide End: 400

CPTAC ID: CPTAC-2848
Peptide Molecular Mass: 2,422.0709
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend