MST1R, CPTAC-2851 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MST1R	PTK8, RON

Sequence Length (AA)	Molecular Weight (Da)
1400	152271

Protein Name
Macrophage-stimulating protein receptor

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MELLPPLPQS	FLLLLLLPAK	PAAGEDWQCP	RTPYAASRDF	DVKYVVPSFS
60	70	80	90	100
AGGLVQAMVT	YEGDRNESAV	FVAIRNRLHV	LGPDLKSVQS	LATGPAGDPG
110	120	130	140	150
CQTCAACGPG	PHGPPGDTDT	KVLVLDPALP	ALVSCGSSLQ	GRCFLHDLEP
160	170	180	190	200
QGTAVHLAAP	ACLFSAHHNR	PDDCPDCVAS	PLGTRVTVVE	QGQASYFYVA
210	220	230	240	250
SSLDAAVAAS	FSPRSVSIRR	LKADASGFAP	GFVALSVLPK	HLVSYSIEYV
260	270	280	290	300
HSFHTGAFVY	FLTVQPASVT	DDPSALHTRL	ARLSATEPEL	GDYRELVLDC
310	320	330	340	350
RFAPKRRRRG	APEGGQPYPV	LRVAHSAPVG	AQLATELSIA	EGQEVLFGVF
360	370	380	390	400
VTGKDGGPGV	GPNSVVCAFP	IDLLDTLIDE	GVERCCESPV	HPGLRRGLDF
410	420	430	440	450
FQSPSFCPNP	PGLEALSPNT	SCRHFPLLVS	SSFSRVDLFN	GLLGPVQVTA
460	470	480	490	500
LYVTRLDNVT	VAHMGTMDGR	ILQVELVRSL	NYLLYVSNFS	LGDSGQPVQR
510	520	530	540	550
DVSRLGDHLL	FASGDQVFQV	PIQGPGCRHF	LTCGRCLRAW	HFMGCGWCGN
560	570	580	590	600
MCGQQKECPG	SWQQDHCPPK	LTEFHPHSGP	LRGSTRLTLC	GSNFYLHPSG
610	620	630	640	650
LVPEGTHQVT	VGQSPCRPLP	KDSSKLRPVP	RKDFVEEFEC	ELEPLGTQAV
660	670	680	690	700
GPTNVSLTVT	NMPPGKHFRV	DGTSVLRGFS	FMEPVLIAVQ	PLFGPRAGGT
710	720	730	740	750
CLTLEGQSLS	VGTSRAVLVN	GTECLLARVS	EGQLLCATPP	GATVASVPLS
760	770	780	790	800
LQVGGAQVPG	SWTFQYREDP	VVLSISPNCG	YINSHITICG	QHLTSAWHLV
810	820	830	840	850
LSFHDGLRAV	ESRCERQLPE	QQLCRLPEYV	VRDPQGWVAG	NLSARGDGAA
860	870	880	890	900
GFTLPGFRFL	PPPHPPSANL	VPLKPEEHAI	KFEYIGLGAV	ADCVGINVTV
910	920	930	940	950
GGESCQHEFR	GDMVVCPLPP	SLQLGQDGAP	LQVCVDGECH	ILGRVVRPGP
960	970	980	990	1000
DGVPQSTLLG	ILLPLLLLVA	ALATALVFSY	WWRRKQLVLP	PNLNDLASLD
1010	1020	1030	1040	1050
QTAGATPLPI	LYSGSDYRSG	LALPAIDGLD	STTCVHGASF	SDSEDESCVP
1060	1070	1080	1090	1100
LLRKESIQLR	DLDSALLAEV	KDVLIPHERV	VTHSDRVIGK	GHFGVVYHGE
1110	1120	1130	1140	1150
YIDQAQNRIQ	CAIKSLSRIT	EMQQVEAFLR	EGLLMRGLNH	PNVLALIGIM
1160	1170	1180	1190	1200
LPPEGLPHVL	LPYMCHGDLL	QFIRSPQRNP	TVKDLISFGL	QVARSMEYLA
1210	1220	1230	1240	1250
EQKFVHRDLA	ARNCMLDESF	TVKVADFGLA	RDILDREYYS	VQQHRHARLP
1260	1270	1280	1290	1300
VKWMALESLQ	TYRFTTKSDV	WSFGVLLWEL	LTRGAPPYRH	IDPFDLTHFL
1310	1320	1330	1340	1350
AQGRRLPQPE	YCPDSLYQVM	QQCWEADPAV	RPTFRVLVGE	VEQIVSALLG
1360	1370	1380	1390	1400
DHYVQLPATY	MNLGPSTSHE	MNVRPEQPQF	SPMPGNVRRP	RPLSEPPRPT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2851 Collapse assay details

Data source: Panorama

Official Gene Symbol: MST1R
Peptide Sequence: GAPEGGQPYPVLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 310
Peptide End: 322

CPTAC ID: CPTAC-2851
Peptide Molecular Mass: 1,339.6885
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	3.2	2.9	2.5	6.8	3.8	2.5	7.5	4.8	3.5	15	15	15
y9 (1+)	3.2	3.9	2.5	6.5	5.2	2.4	7.2	6.5	3.5	15	15	15
b6 (1+)	3.2	3.5	2.3	5.6	4	2.9	6.4	5.3	3.7	15	15	15
sum	2.9	2.9	2.3	6.4	4	2.3	7	4.9	3.3	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2850 Collapse assay details

Data source: Panorama

Official Gene Symbol: MST1R
Peptide Modified Sequence: QLPEQQLC[+57.0]R
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 825
Peptide - Site of Modification: 8
Peptide Start: 817
Peptide End: 825

CPTAC ID: CPTAC-2850
Peptide Molecular Mass: 1,170.5815
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend