ITK, CPTAC-2863 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ITK	EMT, LYK

Sequence Length (AA)	Molecular Weight (Da)
620	71831

Protein Name
Tyrosine-protein kinase ITK/TSK

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MNNFILLEEQ	LIKKSQQKRR	TSPSNFKVRF	FVLTKASLAY	FEDRHGKKRT
60	70	80	90	100
LKGSIELSRI	KCVEIVKSDI	SIPCHYKYPF	QVVHDNYLLY	VFAPDRESRQ
110	120	130	140	150
RWVLALKEET	RNNNSLVPKY	HPNFWMDGKW	RCCSQLEKLA	TGCAQYDPTK
160	170	180	190	200
NASKKPLPPT	PEDNRRPLWE	PEETVVIALY	DYQTNDPQEL	ALRRNEEYCL
210	220	230	240	250
LDSSEIHWWR	VQDRNGHEGY	VPSSYLVEKS	PNNLETYEWY	NKSISRDKAE
260	270	280	290	300
KLLLDTGKEG	AFMVRDSRTA	GTYTVSVFTK	AVVSENNPCI	KHYHIKETND
310	320	330	340	350
NPKRYYVAEK	YVFDSIPLLI	NYHQHNGGGL	VTRLRYPVCF	GRQKAPVTAG
360	370	380	390	400
LRYGKWVIDP	SELTFVQEIG	SGQFGLVHLG	YWLNKDKVAI	KTIREGAMSE
410	420	430	440	450
EDFIEEAEVM	MKLSHPKLVQ	LYGVCLEQAP	ICLVFEFMEH	GCLSDYLRTQ
460	470	480	490	500
RGLFAAETLL	GMCLDVCEGM	AYLEEACVIH	RDLAARNCLV	GENQVIKVSD
510	520	530	540	550
FGMTRFVLDD	QYTSSTGTKF	PVKWASPEVF	SFSRYSSKSD	VWSFGVLMWE
560	570	580	590	600
VFSEGKIPYE	NRSNSEVVED	ISTGFRLYKP	RLASTHVYQI	MNHCWKERPE
610	620
DRPAFSRLLR	QLAEIAESGL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2863 Collapse assay details

Data source: Panorama

Official Gene Symbol: ITK
Peptide Modified Sequence: LATGC[+57.0]AQYDPTK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 144
Peptide - Site of Modification: 5
Peptide Start: 139
Peptide End: 150

CPTAC ID: CPTAC-2863
Peptide Molecular Mass: 1,323.6129
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	3.8	3.2	3.3	5.8	3	3.2	6.9	4.4	4.6	15	15	15
y10 (1+)	4.6	2.9	3.6	5.4	3.4	4.1	7.1	4.5	5.5	15	15	15
y3 (1+)	2.3	2.6	2.6	6.4	2.5	3.3	6.8	3.6	4.2	15	15	15
sum	2.8	2.5	2.5	5.5	2.5	2.8	6.2	3.5	3.8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2862 Collapse assay details

Data source: Panorama

Official Gene Symbol: ITK
Peptide Sequence: TAGTYTVSVFTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 269
Peptide End: 280

CPTAC ID: CPTAC-2862
Peptide Molecular Mass: 1,273.6554
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend