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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MAPK7

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAPK7 BMK1, ERK5, PRKM7
Sequence Length (AA) Molecular Weight (Da)
816 88386
Protein Name
Mitogen-activated protein kinase 7
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAEPLKEEDG EDGSAEPPGP VKAEPAHTAA SVAAKNLALL KARSFDVTFD
60 70 80 90 100
VGDEYEIIET IGNGAYGVVS SARRRLTGQQ VAIKKIPNAF DVVTNAKRTL
110 120 130 140 150
RELKILKHFK HDNIIAIKDI LRPTVPYGEF KSVYVVLDLM ESDLHQIIHS
160 170 180 190 200
SQPLTLEHVR YFLYQLLRGL KYMHSAQVIH RDLKPSNLLV NENCELKIGD
210 220 230 240 250
FGMARGLCTS PAEHQYFMTE YVATRWYRAP ELMLSLHEYT QAIDLWSVGC
260 270 280 290 300
IFGEMLARRQ LFPGKNYVHQ LQLIMMVLGT PSPAVIQAVG AERVRAYIQS
310 320 330 340 350
LPPRQPVPWE TVYPGADRQA LSLLGRMLRF EPSARISAAA ALRHPFLAKY
360 370 380 390 400
HDPDDEPDCA PPFDFAFDRE ALTRERIKEA IVAEIEDFHA RREGIRQQIR
410 420 430 440 450
FQPSLQPVAS EPGCPDVEMP SPWAPSGDCA MESPPPAPPP CPGPAPDTID
460 470 480 490 500
LTLQPPPPVS EPAPPKKDGA ISDNTKAALK AALLKSLRSR LRDGPSAPLE
510 520 530 540 550
APEPRKPVTA QERQREREEK RRRRQERAKE REKRRQERER KERGAGASGG
560 570 580 590 600
PSTDPLAGLV LSDNDRSLLE RWTRMARPAA PALTSVPAPA PAPTPTPTPV
610 620 630 640 650
QPTSPPPGPV AQPTGPQPQS AGSTSGPVPQ PACPPPGPAP HPTGPPGPIP
660 670 680 690 700
VPAPPQIATS TSLLAAQSLV PPPGLPGSST PGVLPYFPPG LPPPDAGGAP
710 720 730 740 750
QSSMSESPDV NLVTQQLSKS QVEDPLPPVF SGTPKGSGAG YGVGFDLEEF
760 770 780 790 800
LNQSFDMGVA DGPQDGQADS ASLSASLLAD WLEGHGMNPA DIESLQREIQ
810 816
MDSPMLLADL PDLQDP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2871 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK7
Peptide Sequence
DILRPTVPYGEFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
119
Peptide End
131
CPTAC ID
CPTAC-2871
Peptide Molecular Mass
1,533.8191
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.8 6.8 4.4 10.3 7.4 5.7 11.8 10 7.2 15 15 15
b6 (1+) 6.4 6.7 4.8 10.2 7 5.7 12 9.7 7.5 15 15 15
y6 (1+) 4.5 6.2 4 12.5 6.6 5.3 13.3 9.1 6.6 15 15 15
sum 4.5 6.4 4.3 10.7 6.9 5.4 11.6 9.4 6.9 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-887 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK7
Peptide Modified Sequence
DLKPSNLLVNENC[+57.0]ELK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
194
Peptide - Site of Modification
13
Peptide Start
182
Peptide End
197
CPTAC ID
CPTAC-887
Peptide Molecular Mass
1,884.9615
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 15.5 12.3 6.6 17.7 14.6 11.7 23.5 19.1 13.4 15 15 15
y5 (1+) 17.7 9.5 9.8 18.8 12.9 11.7 25.8 16 15.3 15 15 15
y6 (1+) 22.8 7.7 12.1 27.4 9 16.2 35.6 11.8 20.2 15 15 15
y7 (1+) 7.2 4.9 4.4 9.3 4.7 7 11.8 6.8 8.3 15 15 15
y8 (1+) 8.8 9.2 9.6 9.5 12.3 9.1 12.9 15.4 13.2 15 15 15
b8 (1+) 24.5 8.2 16.1 16.4 8.5 15.3 29.5 11.8 22.2 15 15 15
b7 (1+) 9.2 4.3 6 13.5 6.9 9 16.3 8.1 10.8 15 15 15
b6 (1+) 11.4 7.2 6.2 12 8.6 5.8 16.6 11.2 8.5 15 15 15
b5 (1+) 27.5 13.8 7.5 24.8 15.1 14.6 37 20.5 16.4 15 15 15
sum 4.9 2.8 3.2 4.7 3.5 4.4 6.8 4.5 5.4 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for CPTAC-2870 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK7
Peptide Sequence
EEDGEDGSAEPPGPVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
7
Peptide End
22
CPTAC ID
CPTAC-2870
Peptide Molecular Mass
1,611.6900
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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view larger image

Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 2.7 3.8 2.3 5.7 4.8 2.8 6.3 6.1 3.6 15 15 15
y11 (1+) 3.5 3.8 4.2 6.6 5.1 4.4 7.5 6.4 6.1 15 15 15
y5 (1+) 4.7 3.7 2.1 6.7 5 2.5 8.2 6.2 3.3 15 15 15
sum 2.8 3.7 2.3 5.8 4.7 2.8 6.4 6 3.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
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View Protein in Panorama
Comments

Assay Details for CPTAC-888 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK7
Peptide Modified Sequence
GLC[+57.0]TSPAEHQYFMTEYVATR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
208
Peptide - Site of Modification
3
Peptide Start
206
Peptide End
225
CPTAC ID
CPTAC-888
Peptide Molecular Mass
2,360.0566
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 39.7 23.2 10.4 40.7 22.1 7.5 56.9 32 12.8 15 15 15
y6 (1+) 50.5 19.6 8.4 43.7 16.5 13.3 66.8 25.6 15.7 15 15 15
y8 (1+) 30.2 12.3 8.2 37.8 13.1 11.1 48.4 18 13.8 15 15 15
y9 (1+) 24.6 11.1 10.4 31.8 11.4 10.4 40.2 15.9 14.7 15 15 15
y10 (1+) 22.9 10.1 11.9 27.3 9.9 12 35.6 14.1 16.9 15 15 15
y11 (1+) 21.9 11.2 12.6 28.9 13 11.9 36.3 17.2 17.3 15 15 15
sum 21.7 4.1 5.3 21.7 4.8 5.7 30.7 6.3 7.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments