PRKG1, CPTAC-2881 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PRKG1	PRKG1B, PRKGR1A, PRKGR1B

Sequence Length (AA)	Molecular Weight (Da)
671	76364

Protein Name
cGMP-dependent protein kinase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSELEEDFAK	ILMLKEERIK	ELEKRLSEKE	EEIQELKRKL	HKCQSVLPVP
60	70	80	90	100
STHIGPRTTR	AQGISAEPQT	YRSFHDLRQA	FRKFTKSERS	KDLIKEAILD
110	120	130	140	150
NDFMKNLELS	QIQEIVDCMY	PVEYGKDSCI	IKEGDVGSLV	YVMEDGKVEV
160	170	180	190	200
TKEGVKLCTM	GPGKVFGELA	ILYNCTRTAT	VKTLVNVKLW	AIDRQCFQTI
210	220	230	240	250
MMRTGLIKHT	EYMEFLKSVP	TFQSLPEEIL	SKLADVLEET	HYENGEYIIR
260	270	280	290	300
QGARGDTFFI	ISKGTVNVTR	EDSPSEDPVF	LRTLGKGDWF	GEKALQGEDV
310	320	330	340	350
RTANVIAAEA	VTCLVIDRDS	FKHLIGGLDD	VSNKAYEDAE	AKAKYEAEAA
360	370	380	390	400
FFANLKLSDF	NIIDTLGVGG	FGRVELVQLK	SEESKTFAMK	ILKKRHIVDT
410	420	430	440	450
RQQEHIRSEK	QIMQGAHSDF	IVRLYRTFKD	SKYLYMLMEA	CLGGELWTIL
460	470	480	490	500
RDRGSFEDST	TRFYTACVVE	AFAYLHSKGI	IYRDLKPENL	ILDHRGYAKL
510	520	530	540	550
VDFGFAKKIG	FGKKTWTFCG	TPEYVAPEII	LNKGHDISAD	YWSLGILMYE
560	570	580	590	600
LLTGSPPFSG	PDPMKTYNII	LRGIDMIEFP	KKIAKNAANL	IKKLCRDNPS
610	620	630	640	650
ERLGNLKNGV	KDIQKHKWFE	GFNWEGLRKG	TLTPPIIPSV	ASPTDTSNFD
660	670	671
SFPEDNDEPP	PDDNSGWDID	F

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2882 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKG1
Peptide Sequence: AQGISAEPQTYR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 61
Peptide End: 72

CPTAC ID: CPTAC-2882
Peptide Molecular Mass: 1,319.6470
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	3.6	4	3.3	7.5	4.4	4.5	8.3	5.9	5.6	15	15	15
y8 (1+)	3.7	3.3	2.7	7.5	4.2	4.2	8.4	5.3	5	15	15	15
b3 (1+)	3.4	4.1	2.1	6.3	4.7	3.2	7.2	6.2	3.8	15	15	15
sum	3.2	3.4	2.5	7.1	4.3	3.9	7.8	5.5	4.6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2881 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKG1
Peptide Sequence: SVPTFQSLPEEILSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 218
Peptide End: 232

CPTAC ID: CPTAC-2881
Peptide Molecular Mass: 1,673.8876
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend