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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

IKBKE

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
IKBKE IKKE, IKKI, KIAA0151
Sequence Length (AA) Molecular Weight (Da)
716 80462
Protein Name
Inhibitor of nuclear factor kappa-B kinase subunit epsilon
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQSTANYLWH TDDLLGQGAT ASVYKARNKK SGELVAVKVF NTTSYLRPRE
60 70 80 90 100
VQVREFEVLR KLNHQNIVKL FAVEETGGSR QKVLVMEYCS SGSLLSVLES
110 120 130 140 150
PENAFGLPED EFLVVLRCVV AGMNHLRENG IVHRDIKPGN IMRLVGEEGQ
160 170 180 190 200
SIYKLTDFGA ARELDDDEKF VSVYGTEEYL HPDMYERAVL RKPQQKAFGV
210 220 230 240 250
TVDLWSIGVT LYHAATGSLP FIPFGGPRRN KEIMYRITTE KPAGAIAGAQ
260 270 280 290 300
RRENGPLEWS YTLPITCQLS LGLQSQLVPI LANILEVEQA KCWGFDQFFA
310 320 330 340 350
ETSDILQRVV VHVFSLSQAV LHHIYIHAHN TIAIFQEAVH KQTSVAPRHQ
360 370 380 390 400
EYLFEGHLCV LEPSVSAQHI AHTTASSPLT LFSTAIPKGL AFRDPALDVP
410 420 430 440 450
KFVPKVDLQA DYNTAKGVLG AGYQALRLAR ALLDGQELMF RGLHWVMEVL
460 470 480 490 500
QATCRRTLEV ARTSLLYLSS SLGTERFSSV AGTPEIQELK AAAELRSRLR
510 520 530 540 550
TLAEVLSRCS QNITETQESL SSLNRELVKS RDQVHEDRSI QQIQCCLDKM
560 570 580 590 600
NFIYKQFKKS RMRPGLGYNE EQIHKLDKVN FSHLAKRLLQ VFQEECVQKY
610 620 630 640 650
QASLVTHGKR MRVVHETRNH LRLVGCSVAA CNTEAQGVQE SLSKLLEELS
660 670 680 690 700
HQLLQDRAKG AQASPPPIAP YPSPTRKDLL LHMQELCEGM KLLASDLLDN
710 716
NRIIERLNRV PAPPDV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2885 Collapse assay details

Data source: Panorama

Official Gene Symbol
IKBKE
Peptide Sequence
GVLGAGYQALR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
417
Peptide End
427
CPTAC ID
CPTAC-2885
Peptide Molecular Mass
1,103.6087
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 7.8 7.6 10.5 11.5 10.7 9.6 13.9 13.1 14.2 15 15 15
y9 (1+) 11.7 7 13.3 14.7 11.1 12.7 18.8 13.1 18.4 15 15 15
y6 (1+) 10.4 9.9 10.2 12.2 13.6 10.1 16 16.8 14.4 15 15 15
sum 8.3 8.1 10.6 11.4 11.5 10 14.1 14.1 14.6 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-2829 Collapse assay details

Data source: Panorama

Official Gene Symbol
IKBKE
Peptide Sequence
PAGAIAGAQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
242
Peptide End
251
CPTAC ID
CPTAC-2829
Peptide Molecular Mass
910.4985
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 5.5 12.6 7 9.1 17.2 7.3 10.6 21.3 10.1 15 15 15
y6 (1+) 21.5 12.9 7 28.5 18.3 8.8 35.7 22.4 11.2 15 15 15
b3 (1+) 13.7 12.4 3.8 20.1 17.6 7.1 24.3 21.5 8.1 15 15 15
sum 9.2 11.9 5.1 12.3 17.2 7 15.4 20.9 8.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments