IRAK1, CPTAC-2897 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
IRAK1	IRAK

Sequence Length (AA)	Molecular Weight (Da)
712	76537

Protein Name
Interleukin-1 receptor-associated kinase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGGPGPGEP	AAPGAQHFLY	EVPPWVMCRF	YKVMDALEPA	DWCQFAALIV
60	70	80	90	100
RDQTELRLCE	RSGQRTASVL	WPWINRNARV	ADLVHILTHL	QLLRARDIIT
110	120	130	140	150
AWHPPAPLPS	PGTTAPRPSS	IPAPAEAEAW	SPRKLPSSAS	TFLSPAFPGS
160	170	180	190	200
QTHSGPELGL	VPSPASLWPP	PPSPAPSSTK	PGPESSVSLL	QGARPFPFCW
210	220	230	240	250
PLCEISRGTH	NFSEELKIGE	GGFGCVYRAV	MRNTVYAVKR	LKENADLEWT
260	270	280	290	300
AVKQSFLTEV	EQLSRFRHPN	IVDFAGYCAQ	NGFYCLVYGF	LPNGSLEDRL
310	320	330	340	350
HCQTQACPPL	SWPQRLDILL	GTARAIQFLH	QDSPSLIHGD	IKSSNVLLDE
360	370	380	390	400
RLTPKLGDFG	LARFSRFAGS	SPSQSSMVAR	TQTVRGTLAY	LPEEYIKTGR
410	420	430	440	450
LAVDTDTFSF	GVVVLETLAG	QRAVKTHGAR	TKYLKDLVEE	EAEEAGVALR
460	470	480	490	500
STQSTLQAGL	AADAWAAPIA	MQIYKKHLDP	RPGPCPPELG	LGLGQLACCC
510	520	530	540	550
LHRRAKRRPP	MTQVYERLEK	LQAVVAGVPG	HSEAASCIPP	SPQENSYVSS
560	570	580	590	600
TGRAHSGAAP	WQPLAAPSGA	SAQAAEQLQR	GPNQPVESDE	SLGGLSAALR
610	620	630	640	650
SWHLTPSCPL	DPAPLREAGC	PQGDTAGESS	WGSGPGSRPT	AVEGLALGSS
660	670	680	690	700
ASSSSEPPQI	IINPARQKMV	QKLALYEDGA	LDSLQLLSSS	SLPGLGLEQD
710	712
RQGPEESDEF	QS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2898 Collapse assay details

Data source: Panorama

Official Gene Symbol: IRAK1
Peptide Sequence: GPNQPVESDESLGGLSAALR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 581
Peptide End: 600

CPTAC ID: CPTAC-2898
Peptide Molecular Mass: 1,995.9861
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	6.4	8	9.8	13	11.7	11.7	14.5	14.2	15.3	15	15	15
y7 (1+)	10.2	7.8	9.6	10.6	13.2	11.3	14.7	15.3	14.8	15	15	15
y5 (1+)	7.5	8.2	10.1	12.4	12.3	12.2	14.5	14.8	15.8	15	15	15
sum	6.5	7.8	9.8	11.8	12	11.6	13.5	14.3	15.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2897 Collapse assay details

Data source: Panorama

Official Gene Symbol: IRAK1
Peptide Sequence: TASVLWPWINR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 66
Peptide End: 76

CPTAC ID: CPTAC-2897
Peptide Molecular Mass: 1,341.7194
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend