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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PRKX PKX1
Sequence Length (AA) Molecular Weight (Da)
358 40896
Protein Name
cAMP-dependent protein kinase catalytic subunit PRKX
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEAPGLAQAA AAESDSRKVA EETPDGAPAL CPSPEALSPE PPVYSLQDFD
60 70 80 90 100
TLATVGTGTF GRVHLVKEKT AKHFFALKVM SIPDVIRLKQ EQHVHNEKSV
110 120 130 140 150
LKEVSHPFLI RLFWTWHDER FLYMLMEYVP GGELFSYLRN RGRFSSTTGL
160 170 180 190 200
FYSAEIICAI EYLHSKEIVY RDLKPENILL DRDGHIKLTD FGFAKKLVDR
210 220 230 240 250
TWTLCGTPEY LAPEVIQSKG HGRAVDWWAL GILIFEMLSG FPPFFDDNPF
260 270 280 290 300
GIYQKILAGK IDFPRHLDFH VKDLIKKLLV VDRTRRLGNM KNGANDVKHH
310 320 330 340 350
RWFRSVDWEA VPQRKLKPPI VPKIAGDGDT SNFETYPEND WDTAAPVPQK
358
DLEIFKNF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2902 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRKX
Peptide Sequence
LFWTWHDER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
112
Peptide End
120
CPTAC ID
CPTAC-2902
Peptide Molecular Mass
1,288.5989
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 4.2 2.9 4.3 8.5 11.4 6.4 9.5 11.8 7.7 15 15 15
y4 (1+) 5.9 3.1 5 8 11.4 6.9 9.9 11.8 8.5 15 15 15
y5 (1+) 3.3 3 4.3 8.5 11.9 6.1 9.1 12.3 7.5 15 15 15
sum 3.9 2.6 4.4 8.1 11.5 6.3 9 11.8 7.7 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2901 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRKX
Peptide Sequence
SVDWEAVPQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
305
Peptide End
314
CPTAC ID
CPTAC-2901
Peptide Molecular Mass
1,185.5778
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 9.2 12.3 7.4 8.4 13.6 7.2 12.5 18.3 10.3 15 15 15
y3 (1+) 7.5 10.8 7.3 7.5 12.7 6.9 10.6 16.7 10 15 15 15
y5 (1+) 10.1 11.2 7.4 10.5 12.9 7.3 14.6 17.1 10.4 15 15 15
sum 8.3 11.3 6.7 8 12.9 6.8 11.5 17.1 9.5 15 15 15


Additional Resources and Comments