PRKX, CPTAC-2902 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PRKX	PKX1

Sequence Length (AA)	Molecular Weight (Da)
358	40896

Protein Name
cAMP-dependent protein kinase catalytic subunit PRKX

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEAPGLAQAA	AAESDSRKVA	EETPDGAPAL	CPSPEALSPE	PPVYSLQDFD
60	70	80	90	100
TLATVGTGTF	GRVHLVKEKT	AKHFFALKVM	SIPDVIRLKQ	EQHVHNEKSV
110	120	130	140	150
LKEVSHPFLI	RLFWTWHDER	FLYMLMEYVP	GGELFSYLRN	RGRFSSTTGL
160	170	180	190	200
FYSAEIICAI	EYLHSKEIVY	RDLKPENILL	DRDGHIKLTD	FGFAKKLVDR
210	220	230	240	250
TWTLCGTPEY	LAPEVIQSKG	HGRAVDWWAL	GILIFEMLSG	FPPFFDDNPF
260	270	280	290	300
GIYQKILAGK	IDFPRHLDFH	VKDLIKKLLV	VDRTRRLGNM	KNGANDVKHH
310	320	330	340	350
RWFRSVDWEA	VPQRKLKPPI	VPKIAGDGDT	SNFETYPEND	WDTAAPVPQK
358
DLEIFKNF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2902 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKX
Peptide Sequence: LFWTWHDER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 112
Peptide End: 120

CPTAC ID: CPTAC-2902
Peptide Molecular Mass: 1,288.5989
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	4.2	2.9	4.3	8.5	11.4	6.4	9.5	11.8	7.7	15	15	15
y4 (1+)	5.9	3.1	5	8	11.4	6.9	9.9	11.8	8.5	15	15	15
y5 (1+)	3.3	3	4.3	8.5	11.9	6.1	9.1	12.3	7.5	15	15	15
sum	3.9	2.6	4.4	8.1	11.5	6.3	9	11.8	7.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2901 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKX
Peptide Sequence: SVDWEAVPQR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 305
Peptide End: 314

CPTAC ID: CPTAC-2901
Peptide Molecular Mass: 1,185.5778
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend