Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

JAK3

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
JAK3 N/A
Sequence Length (AA) Molecular Weight (Da)
1124 125099
Protein Name
Tyrosine-protein kinase JAK3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPPSEETPL IPQRSCSLLS TEAGALHVLL PARGPGPPQR LSFSFGDHLA
60 70 80 90 100
EDLCVQAAKA SGILPVYHSL FALATEDLSC WFPPSHIFSV EDASTQVLLY
110 120 130 140 150
RIRFYFPNWF GLEKCHRFGL RKDLASAILD LPVLEHLFAQ HRSDLVSGRL
160 170 180 190 200
PVGLSLKEQG ECLSLAVLDL ARMAREQAQR PGELLKTVSY KACLPPSLRD
210 220 230 240 250
LIQGLSFVTR RRIRRTVRRA LRRVAACQAD RHSLMAKYIM DLERLDPAGA
260 270 280 290 300
AETFHVGLPG ALGGHDGLGL LRVAGDGGIA WTQGEQEVLQ PFCDFPEIVD
310 320 330 340 350
ISIKQAPRVG PAGEHRLVTV TRTDNQILEA EFPGLPEALS FVALVDGYFR
360 370 380 390 400
LTTDSQHFFC KEVAPPRLLE EVAEQCHGPI TLDFAINKLK TGGSRPGSYV
410 420 430 440 450
LRRSPQDFDS FLLTVCVQNP LGPDYKGCLI RRSPTGTFLL VGLSRPHSSL
460 470 480 490 500
RELLATCWDG GLHVDGVAVT LTSCCIPRPK EKSNLIVVQR GHSPPTSSLV
510 520 530 540 550
QPQSQYQLSQ MTFHKIPADS LEWHENLGHG SFTKIYRGCR HEVVDGEARK
560 570 580 590 600
TEVLLKVMDA KHKNCMESFL EAASLMSQVS YRHLVLLHGV CMAGDSTMVQ
610 620 630 640 650
EFVHLGAIDM YLRKRGHLVP ASWKLQVVKQ LAYALNYLED KGLPHGNVSA
660 670 680 690 700
RKVLLAREGA DGSPPFIKLS DPGVSPAVLS LEMLTDRIPW VAPECLREAQ
710 720 730 740 750
TLSLEADKWG FGATVWEVFS GVTMPISALD PAKKLQFYED RQQLPAPKWT
760 770 780 790 800
ELALLIQQCM AYEPVQRPSF RAVIRDLNSL ISSDYELLSD PTPGALAPRD
810 820 830 840 850
GLWNGAQLYA CQDPTIFEER HLKYISQLGK GNFGSVELCR YDPLGDNTGA
860 870 880 890 900
LVAVKQLQHS GPDQQRDFQR EIQILKALHS DFIVKYRGVS YGPGRQSLRL
910 920 930 940 950
VMEYLPSGCL RDFLQRHRAR LDASRLLLYS SQICKGMEYL GSRRCVHRDL
960 970 980 990 1000
AARNILVESE AHVKIADFGL AKLLPLDKDY YVVREPGQSP IFWYAPESLS
1010 1020 1030 1040 1050
DNIFSRQSDV WSFGVVLYEL FTYCDKSCSP SAEFLRMMGC ERDVPALCRL
1060 1070 1080 1090 1100
LELLEEGQRL PAPPACPAEV HELMKLCWAP SPQDRPSFSA LGPQLDMLWS
1110 1120 1124
GSRGCETHAF TAHPEGKHHS LSFS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-2906 Collapse assay details

Data source: Panorama

Official Gene Symbol
JAK3
Peptide Modified Sequence
EQGEC[+57.0]LSLAVLDLAR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
163
Peptide - Site of Modification
5
Peptide Start
158
Peptide End
172
CPTAC ID
CPTAC-2906
Peptide Molecular Mass
1,672.8454
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 5.2 4.4 5.3 7.2 7.9 6.9 8.9 9 8.7 15 15 15
y3 (1+) 6.2 4.2 5.5 7.5 7.6 6.2 9.7 8.7 8.3 15 15 15
y9 (1+) 5.9 4.5 5.8 7.2 8.1 7.5 9.3 9.3 9.5 15 15 15
sum 5.4 4.3 5.5 7 7.9 7 8.8 9 8.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-2905 Collapse assay details

Data source: Panorama

Official Gene Symbol
JAK3
Peptide Modified Sequence
LTTDSQHFFC[+57.0]K
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
361
Peptide - Site of Modification
10
Peptide Start
351
Peptide End
361
CPTAC ID
CPTAC-2905
Peptide Molecular Mass
1,382.6289
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.7 3.3 5.6 8.7 4 5.4 10.4 5.2 7.8 15 15 15
y4 (1+) 7.1 4.1 4.7 7.9 7.1 4.8 10.6 8.2 6.7 15 15 15
y3 (1+) 4.6 2.8 4.8 5.8 4.9 6.1 7.4 5.6 7.8 15 15 15
sum 4.4 2.4 4.1 6.5 4.7 4.9 7.8 5.3 6.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments