LIMK1, CPTAC-2912 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
LIMK1	LIMK

Sequence Length (AA)	Molecular Weight (Da)
647	72585

Protein Name
LIM domain kinase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRLTLLCCTW	REERMGEEGS	ELPVCASCGQ	RIYDGQYLQA	LNADWHADCF
60	70	80	90	100
RCCDCSASLS	HQYYEKDGQL	FCKKDYWARY	GESCHGCSEQ	ITKGLVMVAG
110	120	130	140	150
ELKYHPECFI	CLTCGTFIGD	GDTYTLVEHS	KLYCGHCYYQ	TVVTPVIEQI
160	170	180	190	200
LPDSPGSHLP	HTVTLVSIPA	SSHGKRGLSV	SIDPPHGPPG	CGTEHSHTVR
210	220	230	240	250
VQGVDPGCMS	PDVKNSIHVG	DRILEINGTP	IRNVPLDEID	LLIQETSRLL
260	270	280	290	300
QLTLEHDPHD	TLGHGLGPET	SPLSSPAYTP	SGEAGSSARQ	KPVLRSCSID
310	320	330	340	350
RSPGAGSLGS	PASQRKDLGR	SESLRVVCRP	HRIFRPSDLI	HGEVLGKGCF
360	370	380	390	400
GQAIKVTHRE	TGEVMVMKEL	IRFDEETQRT	FLKEVKVMRC	LEHPNVLKFI
410	420	430	440	450
GVLYKDKRLN	FITEYIKGGT	LRGIIKSMDS	QYPWSQRVSF	AKDIASGMAY
460	470	480	490	500
LHSMNIIHRD	LNSHNCLVRE	NKNVVVADFG	LARLMVDEKT	QPEGLRSLKK
510	520	530	540	550
PDRKKRYTVV	GNPYWMAPEM	INGRSYDEKV	DVFSFGIVLC	EIIGRVNADP
560	570	580	590	600
DYLPRTMDFG	LNVRGFLDRY	CPPNCPPSFF	PITVRCCDLD	PEKRPSFVKL
610	620	630	640	647
EHWLETLRMH	LAGHLPLGPQ	LEQLDRGFWE	TYRRGESGLP	AHPEVPD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2912 Collapse assay details

Data source: Panorama

Official Gene Symbol: LIMK1
Peptide Modified Sequence: GC[+57.0]FGQAIK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 350
Peptide - Site of Modification: 2
Peptide Start: 348
Peptide End: 355

CPTAC ID: CPTAC-2912
Peptide Molecular Mass: 879.4273
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	5.4	3.4	3	5.6	2.7	3.2	7.8	4.3	4.4	15	14	15
y3 (1+)	6.7	4.2	5.2	10.6	4	6.1	12.5	5.8	8	15	14	15
y5 (1+)	5.6	3.2	2.3	7.6	2.5	3.1	9.4	4.1	3.9	15	14	15
sum	5	3	2.1	5.8	2.3	2.6	7.7	3.8	3.3	15	14	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2911 Collapse assay details

Data source: Panorama

Official Gene Symbol: LIMK1
Peptide Sequence: SPGAGSLGSPASQR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 302
Peptide End: 315

CPTAC ID: CPTAC-2911
Peptide Molecular Mass: 1,270.6266
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend