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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

TAOK3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TAOK3 DPK, JIK, KDS, MAP3K18
Sequence Length (AA) Molecular Weight (Da)
898 105406
Protein Name
Serine/threonine-protein kinase TAO3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRKGVLKDPE IADLFYKDDP EELFIGLHEI GHGSFGAVYF ATNAHTSEVV
60 70 80 90 100
AIKKMSYSGK QTHEKWQDIL KEVKFLRQLK HPNTIEYKGC YLKEHTAWLV
110 120 130 140 150
MEYCLGSASD LLEVHKKPLQ EVEIAAITHG ALHGLAYLHS HALIHRDIKA
160 170 180 190 200
GNILLTEPGQ VKLADFGSAS MASPANSFVG TPYWMAPEVI LAMDEGQYDG
210 220 230 240 250
KVDIWSLGIT CIELAERKPP LFNMNAMSAL YHIAQNDSPT LQSNEWTDSF
260 270 280 290 300
RRFVDYCLQK IPQERPTSAE LLRHDFVRRD RPLRVLIDLI QRTKDAVREL
310 320 330 340 350
DNLQYRKMKK ILFQETRNGP LNESQEDEED SEHGTSLNRE MDSLGSNHSI
360 370 380 390 400
PSMSVSTGSQ SSSVNSMQEV MDESSSELVM MHDDESTINS SSSVVHKKDH
410 420 430 440 450
VFIRDEAGHG DPRPEPRPTQ SVQSQALHYR NRERFATIKS ASLVTRQIHE
460 470 480 490 500
HEQENELREQ MSGYKRMRRQ HQKQLIALEN KLKAEMDEHR LKLQKEVETH
510 520 530 540 550
ANNSSIELEK LAKKQVAIIE KEAKVAAADE KKFQQQILAQ QKKDLTTFLE
560 570 580 590 600
SQKKQYKICK EKIKEEMNED HSTPKKEKQE RISKHKENLQ HTQAEEEAHL
610 620 630 640 650
LTQQRLYYDK NCRFFKRKIM IKRHEVEQQN IREELNKKRT QKEMEHAMLI
660 670 680 690 700
RHDESTRELE YRQLHTLQKL RMDLIRLQHQ TELENQLEYN KRRERELHRK
710 720 730 740 750
HVMELRQQPK NLKAMEMQIK KQFQDTCKVQ TKQYKALKNH QLEVTPKNEH
760 770 780 790 800
KTILKTLKDE QTRKLAILAE QYEQSINEMM ASQALRLDEA QEAECQALRL
810 820 830 840 850
QLQQEMELLN AYQSKIKMQT EAQHERELQK LEQRVSLRRA HLEQKIEEEL
860 870 880 890 898
AALQKERSER IKNLLERQER EIETFDMESL RMGFGNLVTL DFPKEDYR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2950 Collapse assay details

Data source: Panorama

Official Gene Symbol
TAOK3
Peptide Sequence
EVETHANNSSIELEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
496
Peptide End
510
CPTAC ID
CPTAC-2950
Peptide Molecular Mass
1,698.8061
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.2 6 10.6 7.7 23.8 12.3 9.3 24.5 16.2 15 15 15
y4 (1+) 4 8.3 4.9 3.6 11.4 7 5.4 14.1 8.5 15 15 15
y3 (1+) 6.6 11 6.7 6.9 11.7 8.4 9.5 16.1 10.7 15 15 15
sum 3.9 7.1 5.4 4.5 10.5 7.1 6 12.7 8.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-2949 Collapse assay details

Data source: Panorama

Official Gene Symbol
TAOK3
Peptide Sequence
IEEELAALQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
846
Peptide End
855
CPTAC ID
CPTAC-2949
Peptide Molecular Mass
1,142.6183
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 5.4 10.8 8.2 6.7 11.2 8 8.6 15.6 11.5 15 15 15
y5 (1+) 7.9 7.7 7.4 8.4 9.4 9.2 11.5 12.2 11.8 15 15 15
y8 (1+) 10.9 8.8 4.9 10.2 8.9 7 14.9 12.5 8.5 15 15 15
sum 7.7 8.7 6 7.6 9.3 7.7 10.8 12.7 9.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments