EIF2AK4, CPTAC-2963 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
EIF2AK4	GCN2, KIAA1338

Sequence Length (AA)	Molecular Weight (Da)
1649	186911

Protein Name
eIF-2-alpha kinase GCN2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGGRGAPGR	GRDEPPESYP	QRQDHELQAL	EAIYGADFQD	LRPDACGPVK
60	70	80	90	100
EPPEINLVLY	PQGLTGEEVY	VKVDLRVKCP	PTYPDVVPEI	ELKNAKGLSN
110	120	130	140	150
ESVNLLKSRL	EELAKKHCGE	VMIFELAYHV	QSFLSEHNKP	PPKSFHEEML
160	170	180	190	200
ERRAQEEQQR	LLEAKRKEEQ	EQREILHEIQ	RRKEEIKEEK	KRKEMAKQER
210	220	230	240	250
LEIASLSNQD	HTSKKDPGGH	RTAAILHGGS	PDFVGNGKHR	ANSSGRSRRE
260	270	280	290	300
RQYSVCNSED	SPGSCEILYF	NMGSPDQLMV	HKGKCIGSDE	QLGKLVYNAL
310	320	330	340	350
ETATGGFVLL	YEWVLQWQKK	MGPFLTSQEK	EKIDKCKKQI	QGTETEFNSL
360	370	380	390	400
VKLSHPNVVR	YLAMNLKEQD	DSIVVDILVE	HISGVSLAAH	LSHSGPIPVH
410	420	430	440	450
QLRRYTAQLL	SGLDYLHSNS	VVHKVLSASN	VLVDAEGTVK	ITDYSISKRL
460	470	480	490	500
ADICKEDVFE	QTRVRFSDNA	LPYKTGKKGD	VWRLGLLLLS	LSQGQECGEY
510	520	530	540	550
PVTIPSDLPA	DFQDFLKKCV	CLDDKERWSP	QQLLKHSFIN	PQPKMPLVEQ
560	570	580	590	600
SPEDSEGQDY	VETVIPSNRL	PSAAFFSETQ	RQFSRYFIEF	EELQLLGKGA
610	620	630	640	650
FGAVIKVQNK	LDGCCYAVKR	IPINPASRQF	RRIKGEVTLL	SRLHHENIVR
660	670	680	690	700
YYNAWIERHE	RPAGPGTPPP	DSGPLAKDDR	AARGQPASDT	DGLDSVEAAA
710	720	730	740	750
PPPILSSSVE	WSTSGERSAS	ARFPATGPGS	SDDEDDDEDE	HGGVFSQSFL
760	770	780	790	800
PASDSESDII	FDNEDENSKS	QNQDEDCNEK	NGCHESEPSV	TTEAVHYLYI
810	820	830	840	850
QMEYCEKSTL	RDTIDQGLYR	DTVRLWRLFR	EILDGLAYIH	EKGMIHRDLK
860	870	880	890	900
PVNIFLDSDD	HVKIGDFGLA	TDHLAFSADS	KQDDQTGDLI	KSDPSGHLTG
910	920	930	940	950
MVGTALYVSP	EVQGSTKSAY	NQKVDLFSLG	IIFFEMSYHP	MVTASERIFV
960	970	980	990	1000
LNQLRDPTSP	KFPEDFDDGE	HAKQKSVISW	LLNHDPAKRP	TATELLKSEL
1010	1020	1030	1040	1050
LPPPQMEESE	LHEVLHHTLT	NVDGKAYRTM	MAQIFSQRIS	PAIDYTYDSD
1060	1070	1080	1090	1100
ILKGNFSIRT	AKMQQHVCET	IIRIFKRHGA	VQLCTPLLLP	RNRQIYEHNE
1110	1120	1130	1140	1150
AALFMDHSGM	LVMLPFDLRI	PFARYVARNN	ILNLKRYCIE	RVFRPRKLDR
1160	1170	1180	1190	1200
FHPKELLECA	FDIVTSTTNS	FLPTAEIIYT	IYEIIQEFPA	LQERNYSIYL
1210	1220	1230	1240	1250
NHTMLLKAIL	LHCGIPEDKL	SQVYIILYDA	VTEKLTRREV	EAKFCNLSLS
1260	1270	1280	1290	1300
SNSLCRLYKF	IEQKGDLQDL	MPTINSLIKQ	KTGIAQLVKY	GLKDLEEVVG
1310	1320	1330	1340	1350
LLKKLGIKLQ	VLINLGLVYK	VQQHNGIIFQ	FVAFIKRRQR	AVPEILAAGG
1360	1370	1380	1390	1400
RYDLLIPQFR	GPQALGPVPT	AIGVSIAIDK	ISAAVLNMEE	SVTISSCDLL
1410	1420	1430	1440	1450
VVSVGQMSMS	RAINLTQKLW	TAGITAEIMY	DWSQSQEELQ	EYCRHHEITY
1460	1470	1480	1490	1500
VALVSDKEGS	HVKVKSFEKE	RQTEKRVLET	ELVDHVLQKL	RTKVTDERNG
1510	1520	1530	1540	1550
REASDNLAVQ	NLKGSFSNAS	GLFEIHGATV	VPIVSVLAPE	KLSASTRRRY
1560	1570	1580	1590	1600
ETQVQTRLQT	SLANLHQKSS	EIEILAVDLP	KETILQFLSL	EWDADEQAFN
1610	1620	1630	1640	1649
TTVKQLLSRL	PKQRYLKLVC	DEIYNIKVEK	KVSVLFLYSY	RDDYYRILF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2963 Collapse assay details

Data source: Panorama

Official Gene Symbol: EIF2AK4
Peptide Sequence: GLSNESVNLLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 97
Peptide End: 107

CPTAC ID: CPTAC-2963
Peptide Molecular Mass: 1,172.6401
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	6.2	8.9	8.5	6.7	10.1	9.8	9.1	13.5	13	15	15	15
y7 (1+)	17.5	8.8	10.3	19.9	7	11.3	26.5	11.2	15.3	15	15	15
y6 (1+)	9.5	8.4	11.1	9.3	8.7	10.6	13.3	12.1	15.3	15	15	15
sum	7.3	8.4	9.8	8	8.4	10.4	10.8	11.9	14.3	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2962 Collapse assay details

Data source: Panorama

Official Gene Symbol: EIF2AK4
Peptide Sequence: IGDFGLATDHLAFSADSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 864
Peptide End: 881

CPTAC ID: CPTAC-2962
Peptide Molecular Mass: 1,863.9003
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend