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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PI4KB

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PI4KB PIK4CB
Sequence Length (AA) Molecular Weight (Da)
816 91379
Protein Name
Phosphatidylinositol 4-kinase beta
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGDTVVEPAP LKPTSEPTSG PPGNNGGSLL SVITEGVGEL SVIDPEVAQK
60 70 80 90 100
ACQEVLEKVK LLHGGVAVSS RGTPLELVNG DGVDSEIRCL DDPPAQIREE
110 120 130 140 150
EDEMGAAVAS GTAKGARRRR QNNSAKQSWL LRLFESKLFD ISMAISYLYN
160 170 180 190 200
SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL NMYIHMDEDV GDAIKPYIVH
210 220 230 240 250
RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI LSDELKPAHR
260 270 280 290 300
KRELPSLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN
310 320 330 340 350
EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ
360 370 380 390 400
RLISELSLLN HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY
410 420 430 440 450
VEVLECENFD TTSVPARIPE NRIRSTRSVE NLPECGITHE QRAGSFSTVP
460 470 480 490 500
NYDNDDEAWS VDDIGELQVE LPEVHTNSCD NISQFSVDSI TSQESKEPVF
510 520 530 540 550
IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK VRRIREGSPY
560 570 580 590 600
GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI
610 620 630 640 650
LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ
660 670 680 690 700
RNFVQSCAGY CLVCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN
710 720 730 740 750
LGFETSAFKL TTEFVDVMGG LDGDMFNYYK MLMLQGLIAA RKHMDKVVQI
760 770 780 790 800
VEIMQQGSQL PCFHGSSTIR NLKERFHMSM TEEQLQLLVE QMVDGSMRSI
810 816
TTKLYDGFQY LTNGIM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2966 Collapse assay details

Data source: Panorama

Official Gene Symbol
PI4KB
Peptide Sequence
GTPLELVNGDGVDSEIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
72
Peptide End
88
CPTAC ID
CPTAC-2966
Peptide Molecular Mass
1,769.8796
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 5 5 4 5.8 5.6 5.2 7.7 7.5 6.6 15 15 15
y11 (1+) 6.4 5.6 4.1 6.5 6.2 5.4 9.1 8.4 6.8 15 15 15
y10 (1+) 5.2 5.4 4.2 5.7 5.9 5.1 7.7 8 6.6 15 15 15
sum 4.5 5.3 4 5.5 5.8 5.1 7.1 7.9 6.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-2965 Collapse assay details

Data source: Panorama

Official Gene Symbol
PI4KB
Peptide Sequence
SDATASISLSSNLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
277
Peptide End
290
CPTAC ID
CPTAC-2965
Peptide Molecular Mass
1,392.7096
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 15.7 4.1 3.6 17.3 6.5 4.4 23.4 7.7 5.7 15 15 15
y9 (1+) 12.5 3.9 2.9 14.8 4.9 4.3 19.4 6.3 5.2 15 15 15
y10 (1+) 9.7 4.4 4.8 15.1 4.9 6.7 17.9 6.6 8.2 15 15 15
sum 12 3.2 2.8 14.1 4.7 4.1 18.5 5.7 5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments