RPS6KB2, CPTAC-2967 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RPS6KB2	STK14B

Sequence Length (AA)	Molecular Weight (Da)
482	53455

Protein Name
Ribosomal protein S6 kinase beta-2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAVFDLDLE	TEEGSEGEGE	PELSPADACP	LAELRAAGLE	PVGHYEEVEL
60	70	80	90	100
TETSVNVGPE	RIGPHCFELL	RVLGKGGYGK	VFQVRKVQGT	NLGKIYAMKV
110	120	130	140	150
LRKAKIVRNA	KDTAHTRAER	NILESVKHPF	IVELAYAFQT	GGKLYLILEC
160	170	180	190	200
LSGGELFTHL	EREGIFLEDT	ACFYLAEITL	ALGHLHSQGI	IYRDLKPENI
210	220	230	240	250
MLSSQGHIKL	TDFGLCKESI	HEGAVTHTFC	GTIEYMAPEI	LVRSGHNRAV
260	270	280	290	300
DWWSLGALMY	DMLTGSPPFT	AENRKKTMDK	IIRGKLALPP	YLTPDARDLV
310	320	330	340	350
KKFLKRNPSQ	RIGGGPGDAA	DVQRHPFFRH	MNWDDLLAWR	VDPPFRPCLQ
360	370	380	390	400
SEEDVSQFDT	RFTRQTPVDS	PDDTALSESA	NQAFLGFTYV	APSVLDSIKE
410	420	430	440	450
GFSFQPKLRS	PRRLNSSPRA	PVSPLKFSPF	EGFRPSPSLP	EPTELPLPPL
460	470	480	482
LPPPPPSTTA	PLPIRPPSGT	KKSKRGRGRP	GR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2968 Collapse assay details

Data source: Panorama

Official Gene Symbol: RPS6KB2
Peptide Sequence: EGFSFQPK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 400
Peptide End: 407

CPTAC ID: CPTAC-2968
Peptide Molecular Mass: 938.4498
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	6.6	4.2	4	10.6	4.7	3.7	12.5	6.3	5.4	15	15	15
y4 (1+)	7	5.7	3.4	8.4	4.4	3.6	10.9	7.2	5	15	15	15
y3 (1+)	6.2	4.7	2.8	5.8	5	4.4	8.5	6.9	5.2	15	15	15
sum	6.4	4	3.5	8.9	4.2	3.5	11	5.8	4.9	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2967 Collapse assay details

Data source: Panorama

Official Gene Symbol: RPS6KB2
Peptide Sequence: HPFIVELAYAFQTGGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 128
Peptide End: 143

CPTAC ID: CPTAC-2967
Peptide Molecular Mass: 1,776.9199
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend