TBK1, CPTAC-2973 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TBK1	NAK

Sequence Length (AA)	Molecular Weight (Da)
729	83642

Protein Name
Serine/threonine-protein kinase TBK1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MQSTSNHLWL	LSDILGQGAT	ANVFRGRHKK	TGDLFAIKVF	NNISFLRPVD
60	70	80	90	100
VQMREFEVLK	KLNHKNIVKL	FAIEEETTTR	HKVLIMEFCP	CGSLYTVLEE
110	120	130	140	150
PSNAYGLPES	EFLIVLRDVV	GGMNHLRENG	IVHRDIKPGN	IMRVIGEDGQ
160	170	180	190	200
SVYKLTDFGA	ARELEDDEQF	VSLYGTEEYL	HPDMYERAVL	RKDHQKKYGA
210	220	230	240	250
TVDLWSIGVT	FYHAATGSLP	FRPFEGPRRN	KEVMYKIITG	KPSGAISGVQ
260	270	280	290	300
KAENGPIDWS	GDMPVSCSLS	RGLQVLLTPV	LANILEADQE	KCWGFDQFFA
310	320	330	340	350
ETSDILHRMV	IHVFSLQQMT	AHKIYIHSYN	TATIFHELVY	KQTKIISSNQ
360	370	380	390	400
ELIYEGRRLV	LEPGRLAQHF	PKTTEENPIF	VVSREPLNTI	GLIYEKISLP
410	420	430	440	450
KVHPRYDLDG	DASMAKAITG	VVCYACRIAS	TLLLYQELMR	KGIRWLIELI
460	470	480	490	500
KDDYNETVHK	KTEVVITLDF	CIRNIEKTVK	VYEKLMKINL	EAAELGEISD
510	520	530	540	550
IHTKLLRLSS	SQGTIETSLQ	DIDSRLSPGG	SLADAWAHQE	GTHPKDRNVE
560	570	580	590	600
KLQVLLNCMT	EIYYQFKKDK	AERRLAYNEE	QIHKFDKQKL	YYHATKAMTH
610	620	630	640	650
FTDECVKKYE	AFLNKSEEWI	RKMLHLRKQL	LSLTNQCFDI	EEEVSKYQEY
660	670	680	690	700
TNELQETLPQ	KMFTASSGIK	HTMTPIYPSS	NTLVEMTLGM	KKLKEEMEGV
710	720	729
VKELAENNHI	LERFGSLTMD	GGLRNVDCL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-910 Collapse assay details

Data source: Panorama

Official Gene Symbol: TBK1
Peptide Sequence: EPLNTIGLIYEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 385
Peptide End: 396

CPTAC ID: CPTAC-910
Peptide Molecular Mass: 1,388.7551
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	3.4	3.4	2.5	3.7	3.3	3	5	4.7	3.9	15	15	15
y4 (1+)	3.5	3.3	3.5	9.5	3.6	5.3	10.1	4.9	6.4	15	15	15
y5 (1+)	15.2	11.8	6.9	16.2	10.6	6.2	22.2	15.9	9.3	15	15	15
y6 (1+)	4.9	4.6	3.3	3.8	3.7	2.6	6.2	5.9	4.2	15	15	15
y7 (1+)	8.6	4.4	5.1	8.4	5.2	5.4	12	6.8	7.4	15	15	15
y8 (1+)	11.6	4.2	6.9	15.9	4.3	6.8	19.7	6	9.7	15	15	15
y9 (1+)	13.7	6.3	6.5	14.4	6.7	6.9	19.9	9.2	9.5	15	15	15
y10 (1+)	20.5	9	8.6	17.8	9.6	6.8	27.1	13.2	11	15	15	15
sum	2.5	1.6	1.6	3.4	1.7	1.8	4.2	2.3	2.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2973 Collapse assay details

Data source: Panorama

Official Gene Symbol: TBK1
Peptide Sequence: IISSNQELIYEGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 345
Peptide End: 357

CPTAC ID: CPTAC-2973
Peptide Molecular Mass: 1,520.7835
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend