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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TBK1 NAK
Sequence Length (AA) Molecular Weight (Da)
729 83642
Protein Name
Serine/threonine-protein kinase TBK1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQSTSNHLWL LSDILGQGAT ANVFRGRHKK TGDLFAIKVF NNISFLRPVD
60 70 80 90 100
VQMREFEVLK KLNHKNIVKL FAIEEETTTR HKVLIMEFCP CGSLYTVLEE
110 120 130 140 150
PSNAYGLPES EFLIVLRDVV GGMNHLRENG IVHRDIKPGN IMRVIGEDGQ
160 170 180 190 200
SVYKLTDFGA ARELEDDEQF VSLYGTEEYL HPDMYERAVL RKDHQKKYGA
210 220 230 240 250
TVDLWSIGVT FYHAATGSLP FRPFEGPRRN KEVMYKIITG KPSGAISGVQ
260 270 280 290 300
KAENGPIDWS GDMPVSCSLS RGLQVLLTPV LANILEADQE KCWGFDQFFA
310 320 330 340 350
ETSDILHRMV IHVFSLQQMT AHKIYIHSYN TATIFHELVY KQTKIISSNQ
360 370 380 390 400
ELIYEGRRLV LEPGRLAQHF PKTTEENPIF VVSREPLNTI GLIYEKISLP
410 420 430 440 450
KVHPRYDLDG DASMAKAITG VVCYACRIAS TLLLYQELMR KGIRWLIELI
460 470 480 490 500
KDDYNETVHK KTEVVITLDF CIRNIEKTVK VYEKLMKINL EAAELGEISD
510 520 530 540 550
IHTKLLRLSS SQGTIETSLQ DIDSRLSPGG SLADAWAHQE GTHPKDRNVE
560 570 580 590 600
KLQVLLNCMT EIYYQFKKDK AERRLAYNEE QIHKFDKQKL YYHATKAMTH
610 620 630 640 650
FTDECVKKYE AFLNKSEEWI RKMLHLRKQL LSLTNQCFDI EEEVSKYQEY
660 670 680 690 700
TNELQETLPQ KMFTASSGIK HTMTPIYPSS NTLVEMTLGM KKLKEEMEGV
710 720 729
VKELAENNHI LERFGSLTMD GGLRNVDCL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-910 Collapse assay details

Data source: Panorama

Official Gene Symbol
TBK1
Peptide Sequence
EPLNTIGLIYEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
385
Peptide End
396
CPTAC ID
CPTAC-910
Peptide Molecular Mass
1,388.7551
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 3.4 3.4 2.5 3.7 3.3 3 5 4.7 3.9 15 15 15
y4 (1+) 3.5 3.3 3.5 9.5 3.6 5.3 10.1 4.9 6.4 15 15 15
y5 (1+) 15.2 11.8 6.9 16.2 10.6 6.2 22.2 15.9 9.3 15 15 15
y6 (1+) 4.9 4.6 3.3 3.8 3.7 2.6 6.2 5.9 4.2 15 15 15
y7 (1+) 8.6 4.4 5.1 8.4 5.2 5.4 12 6.8 7.4 15 15 15
y8 (1+) 11.6 4.2 6.9 15.9 4.3 6.8 19.7 6 9.7 15 15 15
y9 (1+) 13.7 6.3 6.5 14.4 6.7 6.9 19.9 9.2 9.5 15 15 15
y10 (1+) 20.5 9 8.6 17.8 9.6 6.8 27.1 13.2 11 15 15 15
sum 2.5 1.6 1.6 3.4 1.7 1.8 4.2 2.3 2.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2973 Collapse assay details

Data source: Panorama

Official Gene Symbol
TBK1
Peptide Sequence
IISSNQELIYEGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
345
Peptide End
357
CPTAC ID
CPTAC-2973
Peptide Molecular Mass
1,520.7835
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 8 5.4 3 7.2 3.9 3.4 10.8 6.7 4.5 15 15 15
y4 (1+) 4.9 4.2 2 8.7 3.8 1.7 10 5.7 2.6 15 15 15
y6 (1+) 4.9 4.4 3.5 6.2 3.8 3.7 7.9 5.8 5.1 15 15 15
sum 5 4.5 2.2 7 3.6 2.4 8.6 5.8 3.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2972 Collapse assay details

Data source: Panorama

Official Gene Symbol
TBK1
Peptide Sequence
LAYNEEQIHK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
575
Peptide End
584
CPTAC ID
CPTAC-2972
Peptide Molecular Mass
1,243.6197
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 4.4 5 3 5.2 5.6 2.9 6.8 7.5 4.2 15 15 15
y5 (1+) 8.8 4.2 5.9 10.4 4.7 4.3 13.6 6.3 7.3 15 15 15
y6 (1+) 4.4 5.2 4.4 3.9 6.5 3.9 5.9 8.3 5.9 15 15 15
sum 3.8 3.9 3.5 4.6 5 2.7 6 6.3 4.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-911 Collapse assay details

Data source: Panorama

Official Gene Symbol
TBK1
Peptide Sequence
YQEYTNELQETLPQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
647
Peptide End
661
CPTAC ID
CPTAC-911
Peptide Molecular Mass
1,882.8949
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 5.9 3.6 4.4 6.7 4.4 4.5 8.9 5.7 6.3 15 15 15
y6 (1+) 27.7 10 8.9 26.3 10.6 8.4 38.2 14.6 12.2 15 15 15
b3 (1+) 23.5 8.9 7.5 26.2 11.4 6.3 35.2 14.5 9.8 15 15 15
sum 6.4 2.4 3.5 7.6 4.1 4.1 9.9 4.8 5.4 15 15 15


Additional Resources and Comments