RPS6KA6, CPTAC-2975 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RPS6KA6	RSK4

Sequence Length (AA)	Molecular Weight (Da)
745	83872

Protein Name
Ribosomal protein S6 kinase alpha-6

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLPFAPQDEP	WDREMEVFSG	GGASSGEVNG	LKMVDEPMEE	GEADSCHDEG
60	70	80	90	100
VVKEIPITHH	VKEGYEKADP	AQFELLKVLG	QGSFGKVFLV	RKKTGPDAGQ
110	120	130	140	150
LYAMKVLKKA	SLKVRDRVRT	KMERDILVEV	NHPFIVKLHY	AFQTEGKLYL
160	170	180	190	200
ILDFLRGGDV	FTRLSKEVLF	TEEDVKFYLA	ELALALDHLH	QLGIVYRDLK
210	220	230	240	250
PENILLDEIG	HIKLTDFGLS	KESVDQEKKA	YSFCGTVEYM	APEVVNRRGH
260	270	280	290	300
SQSADWWSYG	VLMFEMLTGT	LPFQGKDRNE	TMNMILKAKL	GMPQFLSAEA
310	320	330	340	350
QSLLRMLFKR	NPANRLGSEG	VEEIKRHLFF	ANIDWDKLYK	REVQPPFKPA
360	370	380	390	400
SGKPDDTFCF	DPEFTAKTPK	DSPGLPASAN	AHQLFKGFSF	VATSIAEEYK
410	420	430	440	450
ITPITSANVL	PIVQINGNAA	QFGEVYELKE	DIGVGSYSVC	KRCIHATTNM
460	470	480	490	500
EFAVKIIDKS	KRDPSEEIEI	LMRYGQHPNI	ITLKDVFDDG	RYVYLVTDLM
510	520	530	540	550
KGGELLDRIL	KQKCFSEREA	SDILYVISKT	VDYLHCQGVV	HRDLKPSNIL
560	570	580	590	600
YMDESASADS	IRICDFGFAK	QLRGENGLLL	TPCYTANFVA	PEVLMQQGYD
610	620	630	640	650
AACDIWSLGV	LFYTMLAGYT	PFANGPNDTP	EEILLRIGNG	KFSLSGGNWD
660	670	680	690	700
NISDGAKDLL	SHMLHMDPHQ	RYTAEQILKH	SWITHRDQLP	NDQPKRNDVS
710	720	730	740	745
HVVKGAMVAT	YSALTHKTFQ	PVLEPVAASS	LAQRRSMKKR	TSTGL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2975 Collapse assay details

Data source: Panorama

Official Gene Symbol: RPS6KA6
Peptide Sequence: GFSFVATSIAEEYK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 387
Peptide End: 400

CPTAC ID: CPTAC-2975
Peptide Molecular Mass: 1,547.7508
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	3.1	3.8	5.7	5.3	7.9	6.2	6.1	8.8	8.4	15	15	15
b3 (1+)	4.3	2.9	3.7	6.9	5.5	4.9	8.1	6.2	6.1	15	15	15
y8 (1+)	4.3	3	4.6	5.2	7.4	5.6	6.7	8	7.2	15	15	15
sum	2.8	3.1	4.8	5	7.1	5.7	5.7	7.7	7.5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2974 Collapse assay details

Data source: Panorama

Official Gene Symbol: RPS6KA6
Peptide Modified Sequence: TVDYLHC[+57.0]QGVVHR
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 537
Peptide - Site of Modification: 7
Peptide Start: 530
Peptide End: 542

CPTAC ID: CPTAC-2974
Peptide Molecular Mass: 1,582.7674
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend