STK11, CPTAC-2999 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STK11	LKB1, PJS

Sequence Length (AA)	Molecular Weight (Da)
433	48636

Protein Name
Serine/threonine-protein kinase STK11

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEVVDPQQLG	MFTEGELMSV	GMDTFIHRID	STEVIYQPRR	KRAKLIGKYL
60	70	80	90	100
MGDLLGEGSY	GKVKEVLDSE	TLCRRAVKIL	KKKKLRRIPN	GEANVKKEIQ
110	120	130	140	150
LLRRLRHKNV	IQLVDVLYNE	EKQKMYMVME	YCVCGMQEML	DSVPEKRFPV
160	170	180	190	200
CQAHGYFCQL	IDGLEYLHSQ	GIVHKDIKPG	NLLLTTGGTL	KISDLGVAEA
210	220	230	240	250
LHPFAADDTC	RTSQGSPAFQ	PPEIANGLDT	FSGFKVDIWS	AGVTLYNITT
260	270	280	290	300
GLYPFEGDNI	YKLFENIGKG	SYAIPGDCGP	PLSDLLKGML	EYEPAKRFSI
310	320	330	340	350
RQIRQHSWFR	KKHPPAEAPV	PIPPSPDTKD	RWRSMTVVPY	LEDLHGADED
360	370	380	390	400
EDLFDIEDDI	IYTQDFTVPG	QVPEEEASHN	GQRRGLPKAV	CMNGTEAAQL
410	420	430	433
STKSRAEGRA	PNPARKACSA	SSKIRRLSAC	KQQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3000 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK11
Peptide Sequence: DIKPGNLLLTTGGTLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 176
Peptide End: 191

CPTAC ID: CPTAC-3000
Peptide Molecular Mass: 1,639.9509
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	12	10.5	11.2	13.9	11.3	11.3	18.4	15.4	15.9	15	15	15
b6 (1+)	14.5	9.9	12.1	14.3	10.7	12.1	20.4	14.6	17.1	15	15	15
y7 (1+)	10.6	9.8	11.1	13.3	10.9	11.8	17	14.7	16.2	15	15	15
sum	12	10.1	11.3	13.5	10.9	11.6	18.1	14.9	16.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2999 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK11
Peptide Sequence: NVIQLVDVLYNEEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 109
Peptide End: 122

CPTAC ID: CPTAC-2999
Peptide Molecular Mass: 1,674.8829
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend