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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

NTRK3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
NTRK3 TRKC
Sequence Length (AA) Molecular Weight (Da)
839 94428
Protein Name
NT-3 growth factor receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDVSLCPAKC SFWRIFLLGS VWLDYVGSVL ACPANCVCSK TEINCRRPDD
60 70 80 90 100
GNLFPLLEGQ DSGNSNGNAS INITDISRNI TSIHIENWRS LHTLNAVDME
110 120 130 140 150
LYTGLQKLTI KNSGLRSIQP RAFAKNPHLR YINLSSNRLT TLSWQLFQTL
160 170 180 190 200
SLRELQLEQN FFNCSCDIRW MQLWQEQGEA KLNSQNLYCI NADGSQLPLF
210 220 230 240 250
RMNISQCDLP EISVSHVNLT VREGDNAVIT CNGSGSPLPD VDWIVTGLQS
260 270 280 290 300
INTHQTNLNW TNVHAINLTL VNVTSEDNGF TLTCIAENVV GMSNASVALT
310 320 330 340 350
VYYPPRVVSL EEPELRLEHC IEFVVRGNPP PTLHWLHNGQ PLRESKIIHV
360 370 380 390 400
EYYQEGEISE GCLLFNKPTH YNNGNYTLIA KNPLGTANQT INGHFLKEPF
410 420 430 440 450
PESTDNFILF DEVSPTPPIT VTHKPEEDTF GVSIAVGLAA FACVLLVVLF
460 470 480 490 500
VMINKYGRRS KFGMKGPVAV ISGEEDSASP LHHINHGITT PSSLDAGPDT
510 520 530 540 550
VVIGMTRIPV IENPQYFRQG HNCHKPDTYV QHIKRRDIVL KRELGEGAFG
560 570 580 590 600
KVFLAECYNL SPTKDKMLVA VKALKDPTLA ARKDFQREAE LLTNLQHEHI
610 620 630 640 650
VKFYGVCGDG DPLIMVFEYM KHGDLNKFLR AHGPDAMILV DGQPRQAKGE
660 670 680 690 700
LGLSQMLHIA SQIASGMVYL ASQHFVHRDL ATRNCLVGAN LLVKIGDFGM
710 720 730 740 750
SRDVYSTDYY RLFNPSGNDF CIWCEVGGHT MLPIRWMPPE SIMYRKFTTE
760 770 780 790 800
SDVWSFGVIL WEIFTYGKQP WFQLSNTEVI ECITQGRVLE RPRVCPKEVY
810 820 830 839
DVMLGCWQRE PQQRLNIKEI YKILHALGKA TPIYLDILG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3002 Collapse assay details

Data source: Panorama

Official Gene Symbol
NTRK3
Peptide Modified Sequence
LNSQNLYC[+57.0]INADGSQLPLFR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
190
Peptide - Site of Modification
8
Peptide Start
182
Peptide End
201
CPTAC ID
CPTAC-3002
Peptide Molecular Mass
2,322.1427
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 5.2 4.6 6.8 5.6 9.7 15.7 7.6 10.7 17.1 15 15 15
y4 (1+) 6.4 3.5 5.1 7 7.2 12.3 9.5 8 13.3 15 15 15
y5 (1+) 7.5 3.1 4.8 8.8 7.8 13.3 11.6 8.4 14.1 15 15 15
sum 5.8 3.5 5.3 6.1 7.9 13.3 8.4 8.6 14.3 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Comments

Assay Details for CPTAC-3001 Collapse assay details

Data source: Panorama

Official Gene Symbol
NTRK3
Peptide Modified Sequence
VFLAEC[+57.0]YNLSPTK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
558
Peptide - Site of Modification
6
Peptide Start
552
Peptide End
564
CPTAC ID
CPTAC-3001
Peptide Molecular Mass
1,540.7596
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 3.2 4.5 4.4 8.2 4.1 4.2 8.8 6.1 6.1 15 15 15
y4 (1+) 2.7 3 2.6 6 3 4.5 6.6 4.2 5.2 15 15 15
y3 (1+) 2.7 3.6 3.3 5.2 3.9 4 5.9 5.3 5.2 15 15 15
sum 2.3 3.7 3.3 6.3 3.4 4 6.7 5 5.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments