STYK1, CPTAC-3013 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STYK1	NOK

Sequence Length (AA)	Molecular Weight (Da)
422	47577

Protein Name
Tyrosine-protein kinase STYK1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGMTRMLLEC	SLSDKLCVIQ	EKQYEVIIVP	TLLVTIFLIL	LGVILWLFIR
60	70	80	90	100
EQRTQQQRSG	PQGIAPVPPP	RDLSWEAGHG	GNVALPLKET	SVENFLGATT
110	120	130	140	150
PALAKLQVPR	EQLSEVLEQI	CSGSCGPIFR	ANMNTGDPSK	PKSVILKALK
160	170	180	190	200
EPAGLHEVQD	FLGRIQFHQY	LGKHKNLVQL	EGCCTEKLPL	YMVLEDVAQG
210	220	230	240	250
DLLSFLWTCR	RDVMTMDGLL	YDLTEKQVYH	IGKQVLLALE	FLQEKHLFHG
260	270	280	290	300
DVAARNILMQ	SDLTAKLCGL	GLAYEVYTRG	AISSTQTIPL	KWLAPERLLL
310	320	330	340	350
RPASIRADVW	SFGILLYEMV	TLGAPPYPEV	PPTSILEHLQ	RRKIMKRPSS
360	370	380	390	400
CTHTMYSIMK	SCWRWREADR	PSPRELRLRL	EAAIKTADDE	AVLQVPELVV
410	420	422
PELYAAVAGI	RVESLFYNYS	ML

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3014 Collapse assay details

Data source: Panorama

Official Gene Symbol: STYK1
Peptide Sequence: EPAGLHEVQDFLGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 151
Peptide End: 164

CPTAC ID: CPTAC-3014
Peptide Molecular Mass: 1,566.7791
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	7.2	7.1	9.4	11	10.6	14.7	13.1	12.8	17.4	15	15	15
y3 (1+)	8.3	7.4	8.5	12.6	11.6	14.8	15.1	13.8	17.1	15	15	15
y4 (1+)	7.5	8.3	8.9	11.3	11.9	14	13.6	14.5	16.6	15	15	15
sum	7.5	7.5	8.9	11.4	11.3	14.5	13.6	13.6	17	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-3013 Collapse assay details

Data source: Panorama

Official Gene Symbol: STYK1
Peptide Modified Sequence: NLVQLEGC[+57.0]C[+57.0]TEK
Modification Type: Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification: 184, 185
Peptide - Site of Modification: 8, 9
Peptide Start: 176
Peptide End: 187

CPTAC ID: CPTAC-3013
Peptide Molecular Mass: 1,449.6592
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend