MARK2, CPTAC-3016 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MARK2	EMK1

Sequence Length (AA)	Molecular Weight (Da)
788	87911

Protein Name
Serine/threonine-protein kinase MARK2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSSARTPLPT	LNERDTEQPT	LGHLDSKPSS	KSNMIRGRNS	ATSADEQPHI
60	70	80	90	100
GNYRLLKTIG	KGNFAKVKLA	RHILTGKEVA	VKIIDKTQLN	SSSLQKLFRE
110	120	130	140	150
VRIMKVLNHP	NIVKLFEVIE	TEKTLYLVME	YASGGEVFDY	LVAHGRMKEK
160	170	180	190	200
EARAKFRQIV	SAVQYCHQKF	IVHRDLKAEN	LLLDADMNIK	IADFGFSNEF
210	220	230	240	250
TFGNKLDTFC	GSPPYAAPEL	FQGKKYDGPE	VDVWSLGVIL	YTLVSGSLPF
260	270	280	290	300
DGQNLKELRE	RVLRGKYRIP	FYMSTDCENL	LKKFLILNPS	KRGTLEQIMK
310	320	330	340	350
DRWMNVGHED	DELKPYVEPL	PDYKDPRRTE	LMVSMGYTRE	EIQDSLVGQR
360	370	380	390	400
YNEVMATYLL	LGYKSSELEG	DTITLKPRPS	ADLTNSSAPS	PSHKVQRSVS
410	420	430	440	450
ANPKQRRFSD	QAAGPAIPTS	NSYSKKTQSN	NAENKRPEED	RESGRKASST
460	470	480	490	500
AKVPASPLPG	LERKKTTPTP	STNSVLSTST	NRSRNSPLLE	RASLGQASIQ
510	520	530	540	550
NGKDSLTMPG	SRASTASASA	AVSAARPRQH	QKSMSASVHP	NKASGLPPTE
560	570	580	590	600
SNCEVPRPST	APQRVPVASP	SAHNISSSGG	APDRTNFPRG	VSSRSTFHAG
610	620	630	640	650
QLRQVRDQQN	LPYGVTPASP	SGHSQGRRGA	SGSIFSKFTS	KFVRRNLSFR
660	670	680	690	700
FARRNLNEPE	SKDRVETLRP	HVVGSGGNDK	EKEEFREAKP	RSLRFTWSMK
710	720	730	740	750
TTSSMEPNEM	MREIRKVLDA	NSCQSELHEK	YMLLCMHGTP	GHEDFVQWEM
760	770	780	788
EVCKLPRLSL	NGVRFKRISG	TSMAFKNIAS	KIANELKL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3016 Collapse assay details

Data source: Panorama

Official Gene Symbol: MARK2
Peptide Sequence: ASLGQASIQNGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 492
Peptide End: 503

CPTAC ID: CPTAC-3016
Peptide Molecular Mass: 1,172.6150
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	5.4	6	5.5	5.2	5	5.6	7.5	7.8	7.8	15	15	15
y6 (1+)	4.1	4.7	5.6	3.1	5.7	5.8	5.1	7.4	8.1	15	15	15
y7 (1+)	5.9	4.9	5	6.3	4.9	5.2	8.6	6.9	7.2	15	15	15
sum	4.3	4.9	5.2	4.1	4.7	5.3	5.9	6.8	7.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3015 Collapse assay details

Data source: Panorama

Official Gene Symbol: MARK2
Peptide Sequence: FLILNPSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 284
Peptide End: 291

CPTAC ID: CPTAC-3015
Peptide Molecular Mass: 930.5539
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend