STK35, CPTAC-3025 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STK35	CLIK1, PDIK1, STK35L1

Sequence Length (AA)	Molecular Weight (Da)
534	58051

Protein Name
Serine/threonine-protein kinase 35

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGHQESPLAR	APAGGAAYVK	RLCKGLSWRE	HVESHGSLGA	QASPASAAAA
60	70	80	90	100
EGSATRRARA	ATSRAARSRR	QPGPGADHPQ	AGAPGGKRAA	RKWRCAGQVT
110	120	130	140	150
IQGPAPPRPR	AGRRDEAGGA	RAAPLLLPPP	PAAMETGKDG	ARRGTQSPER
160	170	180	190	200
KRRSPVPRAP	STKLRPAAAA	RAMDPVAAEA	PGEAFLARRR	PEGGGGSARP
210	220	230	240	250
RYSLLAEIGR	GSYGVVYEAV	AGRSGARVAV	KKIRCDAPEN	VELALAEFWA
260	270	280	290	300
LTSLKRRHQN	VVQFEECVLQ	RNGLAQRMSH	GNKSSQLYLR	LVETSLKGER
310	320	330	340	350
ILGYAEEPCY	LWFVMEFCEG	GDLNQYVLSR	RPDPATNKSF	MLQLTSAIAF
360	370	380	390	400
LHKNHIVHRD	LKPDNILITE	RSGTPILKVA	DFGLSKVCAG	LAPRGKEGNQ
410	420	430	440	450
DNKNVNVNKY	WLSSACGSDF	YMAPEVWEGH	YTAKADIFAL	GIIIWAMIER
460	470	480	490	500
ITFIDSETKK	ELLGTYIKQG	TEIVPVGEAL	LENPKMELHI	PQKRRTSMSE
510	520	530	534
GIKQLLKDML	AANPQDRPDA	FELETRMDQV	TCAA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-3025 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK35
Peptide Sequence: DLKPDNILITER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 360
Peptide End: 371

CPTAC ID: CPTAC-3025
Peptide Molecular Mass: 1,425.7827
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	10.2	6.6	9.6	16.8	7.8	10.1	19.7	10.2	13.9	15	15	15
y5 (1+)	8.8	8	9.8	17.2	9.6	11.7	19.3	12.5	15.3	15	15	15
y4 (1+)	9.3	7.6	9.2	17.3	9.4	10.6	19.6	12.1	14	15	15	15
sum	9.3	7.4	9.4	17.1	9	10.7	19.5	11.7	14.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3024 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK35
Peptide Modified Sequence: VC[+57.0]AGLAPR
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 389
Peptide - Site of Modification: 2
Peptide Start: 387
Peptide End: 394

CPTAC ID: CPTAC-3024
Peptide Molecular Mass: 842.4433
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend