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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

EIF2AK1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
EIF2AK1 HRI, KIAA1369
Sequence Length (AA) Molecular Weight (Da)
630 71106
Protein Name
Eukaryotic translation initiation factor 2-alpha kinase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQGGNSGVRK REEEGDGAGA VAAPPAIDFP AEGPDPEYDE SDVPAEIQVL
60 70 80 90 100
KEPLQQPTFP FAVANQLLLV SLLEHLSHVH EPNPLRSRQV FKLLCQTFIK
110 120 130 140 150
MGLLSSFTCS DEFSSLRLHH NRAITHLMRS AKERVRQDPC EDISRIQKIR
160 170 180 190 200
SREVALEAQT SRYLNEFEEL AILGKGGYGR VYKVRNKLDG QYYAIKKILI
210 220 230 240 250
KGATKTVCMK VLREVKVLAG LQHPNIVGYH TAWIEHVHVI QPRADRAAIE
260 270 280 290 300
LPSLEVLSDQ EEDREQCGVK NDESSSSSII FAEPTPEKEK RFGESDTENQ
310 320 330 340 350
NNKSVKYTTN LVIRESGELE STLELQENGL AGLSASSIVE QQLPLRRNSH
360 370 380 390 400
LEESFTSTEE SSEENVNFLG QTEAQYHLML HIQMQLCELS LWDWIVERNK
410 420 430 440 450
RGREYVDESA CPYVMANVAT KIFQELVEGV FYIHNMGIVH RDLKPRNIFL
460 470 480 490 500
HGPDQQVKIG DFGLACTDIL QKNTDWTNRN GKRTPTHTSR VGTCLYASPE
510 520 530 540 550
QLEGSEYDAK SDMYSLGVVL LELFQPFGTE MERAEVLTGL RTGQLPESLR
560 570 580 590 600
KRCPVQAKYI QHLTRRNSSQ RPSAIQLLQS ELFQNSGNVN LTLQMKIIEQ
610 620 630
EKEIAELKKQ LNLLSQDKGV RDDGKDGGVG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3035 Collapse assay details

Data source: Panorama

Official Gene Symbol
EIF2AK1
Peptide Modified Sequence
IGDFGLAC[+57.0]TDILQK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
467
Peptide - Site of Modification
8
Peptide Start
459
Peptide End
472
CPTAC ID
CPTAC-3035
Peptide Molecular Mass
1,549.7810
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 6.2 2.7 4.4 7 4.7 5.8 9.4 5.4 7.3 15 15 15
y7 (1+) 5.8 4.2 3.8 5.6 6.5 6.3 8.1 7.7 7.4 15 15 15
y8 (1+) 5.8 3.4 3.4 5.5 6.9 6 8 7.7 6.9 15 15 15
sum 4.7 3 3.7 5.2 5.9 5.9 7 6.6 7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments