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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
NTRK1 MTC, TRK, TRKA
Sequence Length (AA) Molecular Weight (Da)
796 87497
Protein Name
High affinity nerve growth factor receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLRGGRRGQL GWHSWAAGPG SLLAWLILAS AGAAPCPDAC CPHGSSGLRC
60 70 80 90 100
TRDGALDSLH HLPGAENLTE LYIENQQHLQ HLELRDLRGL GELRNLTIVK
110 120 130 140 150
SGLRFVAPDA FHFTPRLSRL NLSFNALESL SWKTVQGLSL QELVLSGNPL
160 170 180 190 200
HCSCALRWLQ RWEEEGLGGV PEQKLQCHGQ GPLAHMPNAS CGVPTLKVQV
210 220 230 240 250
PNASVDVGDD VLLRCQVEGR GLEQAGWILT ELEQSATVMK SGGLPSLGLT
260 270 280 290 300
LANVTSDLNR KNVTCWAEND VGRAEVSVQV NVSFPASVQL HTAVEMHHWC
310 320 330 340 350
IPFSVDGQPA PSLRWLFNGS VLNETSFIFT EFLEPAANET VRHGCLRLNQ
360 370 380 390 400
PTHVNNGNYT LLAANPFGQA SASIMAAFMD NPFEFNPEDP IPVSFSPVDT
410 420 430 440 450
NSTSGDPVEK KDETPFGVSV AVGLAVFACL FLSTLLLVLN KCGRRNKFGI
460 470 480 490 500
NRPAVLAPED GLAMSLHFMT LGGSSLSPTE GKGSGLQGHI IENPQYFSDA
510 520 530 540 550
CVHHIKRRDI VLKWELGEGA FGKVFLAECH NLLPEQDKML VAVKALKEAS
560 570 580 590 600
ESARQDFQRE AELLTMLQHQ HIVRFFGVCT EGRPLLMVFE YMRHGDLNRF
610 620 630 640 650
LRSHGPDAKL LAGGEDVAPG PLGLGQLLAV ASQVAAGMVY LAGLHFVHRD
660 670 680 690 700
LATRNCLVGQ GLVVKIGDFG MSRDIYSTDY YRVGGRTMLP IRWMPPESIL
710 720 730 740 750
YRKFTTESDV WSFGVVLWEI FTYGKQPWYQ LSNTEAIDCI TQGRELERPR
760 770 780 790 796
ACPPEVYAIM RGCWQREPQQ RHSIKDVHAR LQALAQAPPV YLDVLG

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3048 Collapse assay details

Data source: Panorama

Official Gene Symbol
NTRK1
Peptide Modified Sequence
NC[+57.0]LVGQGLVVK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
657
Peptide - Site of Modification
2
Peptide Start
655
Peptide End
665
CPTAC ID
CPTAC-3048
Peptide Molecular Mass
1,185.6540
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 6.6 3.8 4.2 16.9 7.5 4.7 18.1 8.4 6.3 15 15 15
y7 (1+) 6.9 3.9 4.3 15.7 6.8 5.4 17.1 7.8 6.9 15 15 15
b3 (1+) 8.9 6 4.5 17.3 7.5 6.9 19.5 9.6 8.2 15 15 15
sum 6.8 3.6 4 16.1 6.9 5.2 17.5 7.8 6.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3047 Collapse assay details

Data source: Panorama

Official Gene Symbol
NTRK1
Peptide Sequence
SGGLPSLGLTLANVTSDLNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
241
Peptide End
260
CPTAC ID
CPTAC-3047
Peptide Molecular Mass
1,984.0589
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6.9 5.5 9.3 9.5 10.3 15 11.7 11.7 17.6 15 15 15
y5 (1+) 7.6 5 8.7 10.4 10 14.6 12.9 11.2 17 15 15 15
b3 (1+) 7.1 5.3 6.5 8.8 8.8 12.4 11.3 10.3 14 15 15 15
sum 7 5.2 8.3 9.5 9.8 14.1 11.8 11.1 16.4 15 15 15


Additional Resources and Comments