PRKCG, CPTAC-3050 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PRKCG	PKCG

Sequence Length (AA)	Molecular Weight (Da)
697	78448

Protein Name
Protein kinase C gamma type

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGLGPGVGD	SEGGPRPLFC	RKGALRQKVV	HEVKSHKFTA	RFFKQPTFCS
60	70	80	90	100
HCTDFIWGIG	KQGLQCQVCS	FVVHRRCHEF	VTFECPGAGK	GPQTDDPRNK
110	120	130	140	150
HKFRLHSYSS	PTFCDHCGSL	LYGLVHQGMK	CSCCEMNVHR	RCVRSVPSLC
160	170	180	190	200
GVDHTERRGR	LQLEIRAPTA	DEIHVTVGEA	RNLIPMDPNG	LSDPYVKLKL
210	220	230	240	250
IPDPRNLTKQ	KTRTVKATLN	PVWNETFVFN	LKPGDVERRL	SVEVWDWDRT
260	270	280	290	300
SRNDFMGAMS	FGVSELLKAP	VDGWYKLLNQ	EEGEYYNVPV	ADADNCSLLQ
310	320	330	340	350
KFEACNYPLE	LYERVRMGPS	SSPIPSPSPS	PTDPKRCFFG	ASPGRLHISD
360	370	380	390	400
FSFLMVLGKG	SFGKVMLAER	RGSDELYAIK	ILKKDVIVQD	DDVDCTLVEK
410	420	430	440	450
RVLALGGRGP	GGRPHFLTQL	HSTFQTPDRL	YFVMEYVTGG	DLMYHIQQLG
460	470	480	490	500
KFKEPHAAFY	AAEIAIGLFF	LHNQGIIYRD	LKLDNVMLDA	EGHIKITDFG
510	520	530	540	550
MCKENVFPGT	TTRTFCGTPD	YIAPEIIAYQ	PYGKSVDWWS	FGVLLYEMLA
560	570	580	590	600
GQPPFDGEDE	EELFQAIMEQ	TVTYPKSLSR	EAVAICKGFL	TKHPGKRLGS
610	620	630	640	650
GPDGEPTIRA	HGFFRWIDWE	RLERLEIPPP	FRPRPCGRSG	ENFDKFFTRA
660	670	680	690	697
APALTPPDRL	VLASIDQADF	QGFTYVNPDF	VHPDARSPTS	PVPVPVM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3050 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKCG
Peptide Sequence: LSVEVWDWDR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 240
Peptide End: 249

CPTAC ID: CPTAC-3050
Peptide Molecular Mass: 1,303.6197
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	2.4	4.3	5.2	6	7.3	13.6	6.5	8.5	14.6	15	15	15
y3 (1+)	4.2	4.5	4	10.7	7.8	13.1	11.5	9	13.7	15	15	15
y6 (1+)	3.1	4.8	5.9	8.6	6.9	13.9	9.1	8.4	15.1	15	15	15
sum	2.8	4.3	5	7.8	7	13.5	8.3	8.2	14.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-3049 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKCG
Peptide Modified Sequence: SVPSLC[+57.0]GVDHTER
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 151
Peptide - Site of Modification: 6
Peptide Start: 145
Peptide End: 157

CPTAC ID: CPTAC-3049
Peptide Molecular Mass: 1,455.6776
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend