YES1, CPTAC-3057 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
YES1	YES

Sequence Length (AA)	Molecular Weight (Da)
543	60801

Protein Name
Tyrosine-protein kinase Yes

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGCIKSKENK	SPAIKYRPEN	TPEPVSTSVS	HYGAEPTTVS	PCPSSSAKGT
60	70	80	90	100
AVNFSSLSMT	PFGGSSGVTP	FGGASSSFSV	VPSSYPAGLT	GGVTIFVALY
110	120	130	140	150
DYEARTTEDL	SFKKGERFQI	INNTEGDWWE	ARSIATGKNG	YIPSNYVAPA
160	170	180	190	200
DSIQAEEWYF	GKMGRKDAER	LLLNPGNQRG	IFLVRESETT	KGAYSLSIRD
210	220	230	240	250
WDEIRGDNVK	HYKIRKLDNG	GYYITTRAQF	DTLQKLVKHY	TEHADGLCHK
260	270	280	290	300
LTTVCPTVKP	QTQGLAKDAW	EIPRESLRLE	VKLGQGCFGE	VWMGTWNGTT
310	320	330	340	350
KVAIKTLKPG	TMMPEAFLQE	AQIMKKLRHD	KLVPLYAVVS	EEPIYIVTEF
360	370	380	390	400
MSKGSLLDFL	KEGDGKYLKL	PQLVDMAAQI	ADGMAYIERM	NYIHRDLRAA
410	420	430	440	450
NILVGENLVC	KIADFGLARL	IEDNEYTARQ	GAKFPIKWTA	PEAALYGRFT
460	470	480	490	500
IKSDVWSFGI	LQTELVTKGR	VPYPGMVNRE	VLEQVERGYR	MPCPQGCPES
510	520	530	540	543
LHELMNLCWK	KDPDERPTFE	YIQSFLEDYF	TATEPQYQPG	ENL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3057 Collapse assay details

Data source: Panorama

Official Gene Symbol: YES1
Peptide Sequence: FQIINNTEGDWWEAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 118
Peptide End: 132

CPTAC ID: CPTAC-3057
Peptide Molecular Mass: 1,877.8697
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	8	3.9	9.1	8.8	10.4	11.7	11.9	11.1	14.8	15	15	15
y5 (1+)	8.7	3.1	7.5	11.2	7.1	9.2	14.2	7.7	11.9	15	15	15
b3 (1+)	9.2	2.9	5.6	8.5	5.7	7.6	12.5	6.4	9.4	15	15	15
sum	7.6	3	7.4	7.7	7.8	9.5	10.8	8.4	12	15	15	15

YES1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-3057 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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YES1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-3057 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments