IGF1R, CPTAC-3061 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
IGF1R	N/A

Sequence Length (AA)	Molecular Weight (Da)
1367	154793

Protein Name
Insulin-like growth factor 1 receptor

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKSGSGGGSP	TSLWGLLFLS	AALSLWPTSG	EICGPGIDIR	NDYQQLKRLE
60	70	80	90	100
NCTVIEGYLH	ILLISKAEDY	RSYRFPKLTV	ITEYLLLFRV	AGLESLGDLF
110	120	130	140	150
PNLTVIRGWK	LFYNYALVIF	EMTNLKDIGL	YNLRNITRGA	IRIEKNADLC
160	170	180	190	200
YLSTVDWSLI	LDAVSNNYIV	GNKPPKECGD	LCPGTMEEKP	MCEKTTINNE
210	220	230	240	250
YNYRCWTTNR	CQKMCPSTCG	KRACTENNEC	CHPECLGSCS	APDNDTACVA
260	270	280	290	300
CRHYYYAGVC	VPACPPNTYR	FEGWRCVDRD	FCANILSAES	SDSEGFVIHD
310	320	330	340	350
GECMQECPSG	FIRNGSQSMY	CIPCEGPCPK	VCEEEKKTKT	IDSVTSAQML
360	370	380	390	400
QGCTIFKGNL	LINIRRGNNI	ASELENFMGL	IEVVTGYVKI	RHSHALVSLS
410	420	430	440	450
FLKNLRLILG	EEQLEGNYSF	YVLDNQNLQQ	LWDWDHRNLT	IKAGKMYFAF
460	470	480	490	500
NPKLCVSEIY	RMEEVTGTKG	RQSKGDINTR	NNGERASCES	DVLHFTSTTT
510	520	530	540	550
SKNRIIITWH	RYRPPDYRDL	ISFTVYYKEA	PFKNVTEYDG	QDACGSNSWN
560	570	580	590	600
MVDVDLPPNK	DVEPGILLHG	LKPWTQYAVY	VKAVTLTMVE	NDHIRGAKSE
610	620	630	640	650
ILYIRTNASV	PSIPLDVLSA	SNSSSQLIVK	WNPPSLPNGN	LSYYIVRWQR
660	670	680	690	700
QPQDGYLYRH	NYCSKDKIPI	RKYADGTIDI	EEVTENPKTE	VCGGEKGPCC
710	720	730	740	750
ACPKTEAEKQ	AEKEEAEYRK	VFENFLHNSI	FVPRPERKRR	DVMQVANTTM
760	770	780	790	800
SSRSRNTTAA	DTYNITDPEE	LETEYPFFES	RVDNKERTVI	SNLRPFTLYR
810	820	830	840	850
IDIHSCNHEA	EKLGCSASNF	VFARTMPAEG	ADDIPGPVTW	EPRPENSIFL
860	870	880	890	900
KWPEPENPNG	LILMYEIKYG	SQVEDQRECV	SRQEYRKYGG	AKLNRLNPGN
910	920	930	940	950
YTARIQATSL	SGNGSWTDPV	FFYVQAKTGY	ENFIHLIIAL	PVAVLLIVGG
960	970	980	990	1000
LVIMLYVFHR	KRNNSRLGNG	VLYASVNPEY	FSAADVYVPD	EWEVAREKIT
1010	1020	1030	1040	1050
MSRELGQGSF	GMVYEGVAKG	VVKDEPETRV	AIKTVNEAAS	MRERIEFLNE
1060	1070	1080	1090	1100
ASVMKEFNCH	HVVRLLGVVS	QGQPTLVIME	LMTRGDLKSY	LRSLRPEMEN
1110	1120	1130	1140	1150
NPVLAPPSLS	KMIQMAGEIA	DGMAYLNANK	FVHRDLAARN	CMVAEDFTVK
1160	1170	1180	1190	1200
IGDFGMTRDI	YETDYYRKGG	KGLLPVRWMS	PESLKDGVFT	TYSDVWSFGV
1210	1220	1230	1240	1250
VLWEIATLAE	QPYQGLSNEQ	VLRFVMEGGL	LDKPDNCPDM	LFELMRMCWQ
1260	1270	1280	1290	1300
YNPKMRPSFL	EIISSIKEEM	EPGFREVSFY	YSEENKLPEP	EELDLEPENM
1310	1320	1330	1340	1350
ESVPLDPSAS	SSSLPLPDRH	SGHKAENGPG	PGVLVLRASF	DERQPYAHMN
1360	1367
GGRKNERALP	LPQSSTC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-3061 Collapse assay details

Data source: Panorama

Official Gene Symbol: IGF1R
Peptide Sequence: AENGPGPGVLVLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 1325
Peptide End: 1337

CPTAC ID: CPTAC-3061
Peptide Molecular Mass: 1,277.7092
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	4	2.6	4.1	10.1	2.9	4.7	10.9	3.9	6.2	15	15	15
y8 (1+)	3.8	3.4	4.9	11	3.1	4.5	11.6	4.6	6.7	15	15	15
y9 (1+)	4.6	2.1	4.5	9.9	2.9	4.5	10.9	3.6	6.4	15	15	15
sum	3.9	2.4	4.3	10.1	2.5	4.4	10.8	3.5	6.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3060 Collapse assay details

Data source: Panorama

Official Gene Symbol: IGF1R
Peptide Sequence: TTINNEYNYR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 195
Peptide End: 204

CPTAC ID: CPTAC-3060
Peptide Molecular Mass: 1,286.5891
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend