Official Gene Symbol | Other Aliases |
---|---|
PLK4 | SAK, STK18 |
Sequence Length (AA) | Molecular Weight (Da) |
---|---|
970 | 108972 |
Protein Name |
---|
Serine/threonine-protein kinase PLK4 |
Sources | |
---|---|
UniProt PhosphoSitePlus ® GeneCards |
Human Protein Atlas |
10 | 20 | 30 | 40 | 50 |
MATCIGEKIE | DFKVGNLLGK | GSFAGVYRAE | SIHTGLEVAI | KMIDKKAMYK |
60 | 70 | 80 | 90 | 100 |
AGMVQRVQNE | VKIHCQLKHP | SILELYNYFE | DSNYVYLVLE | MCHNGEMNRY |
110 | 120 | 130 | 140 | 150 |
LKNRVKPFSE | NEARHFMHQI | ITGMLYLHSH | GILHRDLTLS | NLLLTRNMNI |
160 | 170 | 180 | 190 | 200 |
KIADFGLATQ | LKMPHEKHYT | LCGTPNYISP | EIATRSAHGL | ESDVWSLGCM |
210 | 220 | 230 | 240 | 250 |
FYTLLIGRPP | FDTDTVKNTL | NKVVLADYEM | PSFLSIEAKD | LIHQLLRRNP |
260 | 270 | 280 | 290 | 300 |
ADRLSLSSVL | DHPFMSRNSS | TKSKDLGTVE | DSIDSGHATI | STAITASSST |
310 | 320 | 330 | 340 | 350 |
SISGSLFDKR | RLLIGQPLPN | KMTVFPKNKS | STDFSSSGDG | NSFYTQWGNQ |
360 | 370 | 380 | 390 | 400 |
ETSNSGRGRV | IQDAEERPHS | RYLRRAYSSD | RSGTSNSQSQ | AKTYTMERCH |
410 | 420 | 430 | 440 | 450 |
SAEMLSVSKR | SGGGENEERY | SPTDNNANIF | NFFKEKTSSS | SGSFERPDNN |
460 | 470 | 480 | 490 | 500 |
QALSNHLCPG | KTPFPFADPT | PQTETVQQWF | GNLQINAHLR | KTTEYDSISP |
510 | 520 | 530 | 540 | 550 |
NRDFQGHPDL | QKDTSKNAWT | DTKVKKNSDA | SDNAHSVKQQ | NTMKYMTALH |
560 | 570 | 580 | 590 | 600 |
SKPEIIQQEC | VFGSDPLSEQ | SKTRGMEPPW | GYQNRTLRSI | TSPLVAHRLK |
610 | 620 | 630 | 640 | 650 |
PIRQKTKKAV | VSILDSEEVC | VELVKEYASQ | EYVKEVLQIS | SDGNTITIYY |
660 | 670 | 680 | 690 | 700 |
PNGGRGFPLA | DRPPSPTDNI | SRYSFDNLPE | KYWRKYQYAS | RFVQLVRSKS |
710 | 720 | 730 | 740 | 750 |
PKITYFTRYA | KCILMENSPG | ADFEVWFYDG | VKIHKTEDFI | QVIEKTGKSY |
760 | 770 | 780 | 790 | 800 |
TLKSESEVNS | LKEEIKMYMD | HANEGHRICL | ALESIISEEE | RKTRSAPFFP |
810 | 820 | 830 | 840 | 850 |
IIIGRKPGST | SSPKALSPPP | SVDSNYPTRE | RASFNRMVMH | SAASPTQAPI |
860 | 870 | 880 | 890 | 900 |
LNPSMVTNEG | LGLTTTASGT | DISSNSLKDC | LPKSAQLLKS | VFVKNVGWAT |
910 | 920 | 930 | 940 | 950 |
QLTSGAVWVQ | FNDGSQLVVQ | AGVSSISYTS | PNGQTTRYGE | NEKLPDYIKQ |
960 | 970 | |||
KLQCLSSILL | MFSNPTPNFH |
Data source: UniProt
Uniprot Database Entry PhosphoSitePlus ®
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Data source: Panorama
Data source: Panorama
Data source: Panorama
Data source: Panorama
LOD (fmol) | LLOQ (fmol) | ||||||
---|---|---|---|---|---|---|---|
Peptide | Transition | blank only | blank+low-conc | rsd limit | blank only | blank+low-conc | rsd limit |
HYTLC[+57.0]GTPNYISPEIATR | 3.y6.1 | 2.6e-1 | 2.6e-1 | 2.6e-1 | 2.8e-1 | 3.1e-1 | 3.0e-1 |
3.y7.1 | 5.0e-2 | 5.2e-2 | 5.7e-2 | 5.7e-2 | 6.9e-2 | 8.4e-2 | |
3.y8.1 | NA | NA | -3.0e-1 | NA | NA | -2.9e-1 | |
Sum | 1.2e-1 | 1.2e-1 | 1.1e-1 | 1.3e-1 | 1.5e-1 | 1.2e-1 |
Curve Fit | ||||
---|---|---|---|---|
Peptide | Transition | slope | intercept | r squared |
HYTLC[+57.0]GTPNYISPEIATR | 3.y8.1 | 1.02 | 0.3 | 1 |
3.y7.1 | 1.11 | -0.05 | 1 | |
3.y6.1 | 1.08 | -0.25 | 1 | |
Sum | 1.12 | -0.13 | 1 |
Data source: Panorama
Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
y8 (1+) | 3.8 | 3.4 | 1.8 | 5.8 | 3.5 | 3 | 6.9 | 4.9 | 3.5 | 15 | 15 | 15 |
y6 (1+) | 2.3 | 3 | 2.1 | 6.1 | 2.5 | 2.6 | 6.5 | 3.9 | 3.3 | 15 | 15 | 15 |
y7 (1+) | 3.5 | 3.2 | 2.1 | 6.5 | 3.1 | 3 | 7.4 | 4.5 | 3.7 | 15 | 15 | 15 |
sum | 2.5 | 3.1 | 1.8 | 5.9 | 2.9 | 2.8 | 6.4 | 4.2 | 3.3 | 15 | 15 | 15 |
Data source: Panorama
Data source: Panorama
Data source: Panorama
Data source: Panorama
LOD (fmol) | LLOQ (fmol) | ||||||
---|---|---|---|---|---|---|---|
Peptide | Transition | blank only | blank+low-conc | rsd limit | blank only | blank+low-conc | rsd limit |
IADFGLATQLK | 2.y10.1 | NA | NA | 8.3e-1 | NA | NA | 2.8e+0 |
2.y5.1 | 8.0e-2 | 8.2e-2 | 2.1e-1 | 8.3e-2 | 9.1e-2 | 4.8e-1 | |
2.y7.1 | -8.8e-2 | -8.7e-2 | 9.7e-2 | -8.8e-2 | -8.4e-2 | 4.7e-1 | |
Sum | -5.3e-2 | -5.2e-2 | 1.3e-1 | -5.1e-2 | -4.4e-2 | 5.2e-1 |
Curve Fit | ||||
---|---|---|---|---|
Peptide | Transition | slope | intercept | r squared |
IADFGLATQLK | 2.y7.1 | 1.07 | 0.09 | 1 |
2.y5.1 | 1.1 | -0.08 | 1 | |
2.y10.1 | 1.05 | 0.18 | 1 | |
Sum | 1.07 | 0.06 | 1 |
Data source: Panorama
Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
y10 (1+) | 6.8 | 6.1 | 8.5 | 8.5 | 8.2 | 10.5 | 10.9 | 10.2 | 13.5 | 15 | 15 | 15 |
y7 (1+) | 5.8 | 5.8 | 8.9 | 8.1 | 7.8 | 10.1 | 10 | 9.7 | 13.5 | 15 | 15 | 15 |
y5 (1+) | 7.3 | 6.4 | 7.9 | 8.2 | 7.8 | 9.5 | 11 | 10.1 | 12.4 | 15 | 15 | 15 |
sum | 5.9 | 6 | 8.4 | 7.8 | 7.8 | 9.8 | 9.8 | 9.8 | 12.9 | 15 | 15 | 15 |
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