GAK, CPTAC-3085 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GAK	N/A

Sequence Length (AA)	Molecular Weight (Da)
1311	143191

Protein Name
Cyclin-G-associated kinase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSLLQSALDF	LAGPGSLGGA	SGRDQSDFVG	QTVELGELRL	RVRRVLAEGG
60	70	80	90	100
FAFVYEAQDV	GSGREYALKR	LLSNEEEKNR	AIIQEVCFMK	KLSGHPNIVQ
110	120	130	140	150
FCSAASIGKE	ESDTGQAEFL	LLTELCKGQL	VEFLKKMESR	GPLSCDTVLK
160	170	180	190	200
IFYQTCRAVQ	HMHRQKPPII	HRDLKVENLL	LSNQGTIKLC	DFGSATTISH
210	220	230	240	250
YPDYSWSAQR	RALVEEEITR	NTTPMYRTPE	IIDLYSNFPI	GEKQDIWALG
260	270	280	290	300
CILYLLCFRQ	HPFEDGAKLR	IVNGKYSIPP	HDTQYTVFHS	LIRAMLQVNP
310	320	330	340	350
EERLSIAEVV	HQLQEIAAAR	NVNPKSPITE	LLEQNGGYGS	ATLSRGPPPP
360	370	380	390	400
VGPAGSGYSG	GLALAEYDQP	YGGFLDILRG	GTERLFTNLK	DTSSKVIQSV
410	420	430	440	450
ANYAKGDLDI	SYITSRIAVM	SFPAEGVESA	LKNNIEDVRL	FLDSKHPGHY
460	470	480	490	500
AVYNLSPRTY	RPSRFHNRVS	ECGWAARRAP	HLHTLYNICR	NMHAWLRQDH
510	520	530	540	550
KNVCVVHCMD	GRAASAVAVC	SFLCFCRLFS	TAEAAVYMFS	MKRCPPGIWP
560	570	580	590	600
SHKRYIEYMC	DMVAEEPITP	HSKPILVRAV	VMTPVPLFSK	QRSGCRPFCE
610	620	630	640	650
VYVGDERVAS	TSQEYDKMRD	FKIEDGKAVI	PLGVTVQGDV	LIVIYHARST
660	670	680	690	700
LGGRLQAKMA	SMKMFQIQFH	TGFVPRNATT	VKFAKYDLDA	CDIQEKYPDL
710	720	730	740	750
FQVNLEVEVE	PRDRPSREAP	PWENSSMRGL	NPKILFSSRE	EQQDILSKFG
760	770	780	790	800
KPELPRQPGS	TAQYDAGAGS	PEAEPTDSDS	PPSSSADASR	FLHTLDWQEE
810	820	830	840	850
KEAETGAENA	SSKESESALM	EDRDESEVSD	EGGSPISSEG	QEPRADPEPP
860	870	880	890	900
GLAAGLVQQD	LVFEVETPAV	LPEPVPQEDG	VDLLGLHSEV	GAGPAVPPQA
910	920	930	940	950
CKAPSSNTDL	LSCLLGPPEA	ASQGPPEDLL	SEDPLLLASP	APPLSVQSTP
960	970	980	990	1000
RGGPPAAADP	FGPLLPSSGN	NSQPCSNPDL	FGEFLNSDSV	TVPPSFPSAH
1010	1020	1030	1040	1050
SAPPPSCSAD	FLHLGDLPGE	PSKMTASSSN	PDLLGGWAAW	TETAASAVAP
1060	1070	1080	1090	1100
TPATEGPLFS	PGGQPAPCGS	QASWTKSQNP	DPFADLGDLS	SGLQGSPAGF
1110	1120	1130	1140	1150
PPGGFIPKTA	TTPKGSSSWQ	TSRPPAQGAS	WPPQAKPPPK	ACTQPRPNYA
1160	1170	1180	1190	1200
SNFSVIGARE	ERGVRAPSFA	QKPKVSENDF	EDLLSNQGFS	SRSDKKGPKT
1210	1220	1230	1240	1250
IAEMRKQDLA	KDTDPLKLKL	LDWIEGKERN	IRALLSTLHT	VLWDGESRWT
1260	1270	1280	1290	1300
PVGMADLVAP	EQVKKHYRRA	VLAVHPDKAA	GQPYEQHAKM	IFMELNDAWS
1310	1311
EFENQGSRPL	F

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-3086 Collapse assay details

Data source: Panorama

Official Gene Symbol: GAK
Peptide Modified Sequence: AASAVAVC[+57.0]SFLC[+57.0]FC[+57.0]R
Modification Type: Carbamidomethyl (C), Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification: 521, 525, 527
Peptide - Site of Modification: 8, 12, 14
Peptide Start: 513
Peptide End: 527

CPTAC ID: CPTAC-3086
Peptide Molecular Mass: 1,717.7739
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	12	2.3	2.3	18.7	3.6	6.5	22.2	4.3	6.9	15	15	15
y10 (1+)	10.8	3	3.6	17.5	3.1	7	20.6	4.3	7.9	15	15	15
y8 (1+)	14.1	3	3	18.6	3.2	6.6	23.3	4.4	7.2	15	15	15
sum	11.8	2.7	2.9	17.8	3.1	6.7	21.4	4.1	7.3	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3085 Collapse assay details

Data source: Panorama

Official Gene Symbol: GAK
Peptide Sequence: AVLAVHPDK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 1270
Peptide End: 1278

CPTAC ID: CPTAC-3085
Peptide Molecular Mass: 948.5393
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend