DAPK3, CPTAC-3092 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
DAPK3	ZIPK

Sequence Length (AA)	Molecular Weight (Da)
454	52536

Protein Name
Death-associated protein kinase 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSTFRQEDVE	DHYEMGEELG	SGQFAIVRKC	RQKGTGKEYA	AKFIKKRRLS
60	70	80	90	100
SSRRGVSREE	IEREVNILRE	IRHPNIITLH	DIFENKTDVV	LILELVSGGE
110	120	130	140	150
LFDFLAEKES	LTEDEATQFL	KQILDGVHYL	HSKRIAHFDL	KPENIMLLDK
160	170	180	190	200
NVPNPRIKLI	DFGIAHKIEA	GNEFKNIFGT	PEFVAPEIVN	YEPLGLEADM
210	220	230	240	250
WSIGVITYIL	LSGASPFLGE	TKQETLTNIS	AVNYDFDEEY	FSNTSELAKD
260	270	280	290	300
FIRRLLVKDP	KRRMTIAQSL	EHSWIKAIRR	RNVRGEDSGR	KPERRRLKTT
310	320	330	340	350
RLKEYTIKSH	SSLPPNNSYA	DFERFSKVLE	EAAAAEEGLR	ELQRSRRLCH
360	370	380	390	400
EDVEALAAIY	EEKEAWYREE	SDSLGQDLRR	LRQELLKTEA	LKRQAQEEAK
410	420	430	440	450
GALLGTSGLK	RRFSRLENRY	EALAKQVASE	MRFVQDLVRA	LEQEKLQGVE
454
CGLR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3092 Collapse assay details

Data source: Panorama

Official Gene Symbol: DAPK3
Peptide Sequence: ESLTEDEATQFLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 109
Peptide End: 121

CPTAC ID: CPTAC-3092
Peptide Molecular Mass: 1,509.7199
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	4.4	5.5	4	5.9	5.2	3.7	7.4	7.6	5.4	15	15	15
y8 (1+)	4.2	5.1	3.4	4.6	4.6	3	6.2	6.9	4.5	15	15	15
y9 (1+)	4	5.5	4.4	4.7	5.2	4	6.2	7.6	5.9	15	15	15
sum	3.4	5.1	3.7	4	4.8	3.3	5.2	7	5	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-3091 Collapse assay details

Data source: Panorama

Official Gene Symbol: DAPK3
Peptide Modified Sequence: LC[+57.0]HEDVEALAAIYEEK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 350
Peptide - Site of Modification: 2
Peptide Start: 348
Peptide End: 363

CPTAC ID: CPTAC-3091
Peptide Molecular Mass: 1,888.8877
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend