RIPK2, CPTAC-3094 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RIPK2	CARDIAK, RICK, RIP2

Sequence Length (AA)	Molecular Weight (Da)
540	61195

Protein Name
Receptor-interacting serine/threonine-protein kinase 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MNGEAICSAL	PTIPYHKLAD	LRYLSRGASG	TVSSARHADW	RVQVAVKHLH
60	70	80	90	100
IHTPLLDSER	KDVLREAEIL	HKARFSYILP	ILGICNEPEF	LGIVTEYMPN
110	120	130	140	150
GSLNELLHRK	TEYPDVAWPL	RFRILHEIAL	GVNYLHNMTP	PLLHHDLKTQ
160	170	180	190	200
NILLDNEFHV	KIADFGLSKW	RMMSLSQSRS	SKSAPEGGTI	IYMPPENYEP
210	220	230	240	250
GQKSRASIKH	DIYSYAVITW	EVLSRKQPFE	DVTNPLQIMY	SVSQGHRPVI
260	270	280	290	300
NEESLPYDIP	HRARMISLIE	SGWAQNPDER	PSFLKCLIEL	EPVLRTFEEI
310	320	330	340	350
TFLEAVIQLK	KTKLQSVSSA	IHLCDKKKME	LSLNIPVNHG	PQEESCGSSQ
360	370	380	390	400
LHENSGSPET	SRSLPAPQDN	DFLSRKAQDC	YFMKLHHCPG	NHSWDSTISG
410	420	430	440	450
SQRAAFCDHK	TTPCSSAIIN	PLSTAGNSER	LQPGIAQQWI	QSKREDIVNQ
460	470	480	490	500
MTEACLNQSL	DALLSRDLIM	KEDYELVSTK	PTRTSKVRQL	LDTTDIQGEE
510	520	530	540
FAKVIVQKLK	DNKQMGLQPY	PEILVVSRSP	SLNLLQNKSM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3094 Collapse assay details

Data source: Panorama

Official Gene Symbol: RIPK2
Peptide Sequence: SPSLNLLQNK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 529
Peptide End: 538

CPTAC ID: CPTAC-3094
Peptide Molecular Mass: 1,112.6190
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	7.6	6.1	5.2	6.4	9.4	8	9.9	11.2	9.5	15	15	15
y5 (1+)	12.4	11.6	13.7	13.2	12.2	24.6	18.1	16.8	28.2	15	15	15
b3 (1+)	12.3	4.6	14.9	11.2	8.7	19.7	16.6	9.8	24.7	15	15	15
sum	8.5	4.6	7.8	5.4	8.2	11.6	10.1	9.4	14	15	15	15

RIPK2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-3094 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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RIPK2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-3094 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments