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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ULK1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ULK1 KIAA0722
Sequence Length (AA) Molecular Weight (Da)
1050 112631
Protein Name
Serine/threonine-protein kinase ULK1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEPGRGGTET VGKFEFSRKD LIGHGAFAVV FKGRHREKHD LEVAVKCINK
60 70 80 90 100
KNLAKSQTLL GKEIKILKEL KHENIVALYD FQEMANSVYL VMEYCNGGDL
110 120 130 140 150
ADYLHAMRTL SEDTIRLFLQ QIAGAMRLLH SKGIIHRDLK PQNILLSNPA
160 170 180 190 200
GRRANPNSIR VKIADFGFAR YLQSNMMAAT LCGSPMYMAP EVIMSQHYDG
210 220 230 240 250
KADLWSIGTI VYQCLTGKAP FQASSPQDLR LFYEKNKTLV PTIPRETSAP
260 270 280 290 300
LRQLLLALLQ RNHKDRMDFD EFFHHPFLDA SPSVRKSPPV PVPSYPSSGS
310 320 330 340 350
GSSSSSSSTS HLASPPSLGE MQQLQKTLAS PADTAGFLHS SRDSGGSKDS
360 370 380 390 400
SCDTDDFVMV PAQFPGDLVA EAPSAKPPPD SLMCSGSSLV ASAGLESHGR
410 420 430 440 450
TPSPSPPCSS SPSPSGRAGP FSSSRCGASV PIPVPTQVQN YQRIERNLQS
460 470 480 490 500
PTQFQTPRSS AIRRSGSTSP LGFARASPSP PAHAEHGGVL ARKMSLGGGR
510 520 530 540 550
PYTPSPQVGT IPERPGWSGT PSPQGAEMRG GRSPRPGSSA PEHSPRTSGL
560 570 580 590 600
GCRLHSAPNL SDLHVVRPKL PKPPTDPLGA VFSPPQASPP QPSHGLQSCR
610 620 630 640 650
NLRGSPKLPD FLQRNPLPPI LGSPTKAVPS FDFPKTPSSQ NLLALLARQG
660 670 680 690 700
VVMTPPRNRT LPDLSEVGPF HGQPLGPGLR PGEDPKGPFG RSFSTSRLTD
710 720 730 740 750
LLLKAAFGTQ APDPGSTESL QEKPMEIAPS AGFGGSLHPG ARAGGTSSPS
760 770 780 790 800
PVVFTVGSPP SGSTPPQGPR TRMFSAGPTG SASSSARHLV PGPCSEAPAP
810 820 830 840 850
ELPAPGHGCS FADPITANLE GAVTFEAPDL PEETLMEQEH TEILRGLRFT
860 870 880 890 900
LLFVQHVLEI AALKGSASEA AGGPEYQLQE SVVADQISLL SREWGFAEQL
910 920 930 940 950
VLYLKVAELL SSGLQSAIDQ IRAGKLCLSS TVKQVVRRLN ELYKASVVSC
960 970 980 990 1000
QGLSLRLQRF FLDKQRLLDR IHSITAERLI FSHAVQMVQS AALDEMFQHR
1010 1020 1030 1040 1050
EGCVPRYHKA LLLLEGLQHM LSDQADIENV TKCKLCIERR LSALLTGICA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3099 Collapse assay details

Data source: Panorama

Official Gene Symbol
ULK1
Peptide Modified Sequence
C[+57.0]GASVPIPVPTQVQNYQR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
427
Peptide - Site of Modification
1
Peptide Start
426
Peptide End
443
CPTAC ID
CPTAC-3099
Peptide Molecular Mass
2,013.0102
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 51.4 4.3 9.5 50.7 7 12.6 72.2 8.2 15.8 15 15 15
y5 (1+) 23.7 5.3 10 26.4 4.9 10.9 35.5 7.2 14.8 15 15 15
y9 (1+) 11.7 5.3 6.5 14.3 8 8.3 18.5 9.6 10.5 15 15 15
sum 13.4 4.3 7.1 15.8 6.9 8.9 20.7 8.1 11.4 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Assay Details for CPTAC-3098 Collapse assay details

Data source: Panorama

Official Gene Symbol
ULK1
Peptide Sequence
TLASPADTAGFLHSSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
327
Peptide End
342
CPTAC ID
CPTAC-3098
Peptide Molecular Mass
1,629.8111
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 13.1 8.4 7.6 12.9 8.1 8.5 18.4 11.7 11.4 15 15 15
y7 (1+) 10 7.9 7 12.4 7.4 7.8 15.9 10.8 10.5 15 15 15
y8 (1+) 10.1 11.1 11.9 15.9 11.8 12.3 18.8 16.2 17.1 15 15 15
sum 9.4 8.9 8.5 11.5 8.5 8.5 14.9 12.3 12 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments