TEC, CPTAC-3135 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TEC	PSCTK4

Sequence Length (AA)	Molecular Weight (Da)
631	73581

Protein Name
Tyrosine-protein kinase Tec

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MNFNTILEEI	LIKRSQQKKK	TSPLNYKERL	FVLTKSMLTY	YEGRAEKKYR
60	70	80	90	100
KGFIDVSKIK	CVEIVKNDDG	VIPCQNKYPF	QVVHDANTLY	IFAPSPQSRD
110	120	130	140	150
LWVKKLKEEI	KNNNNIMIKY	HPKFWTDGSY	QCCRQTEKLA	PGCEKYNLFE
160	170	180	190	200
SSIRKALPPA	PETKKRRPPP	PIPLEEEDNS	EEIVVAMYDF	QAAEGHDLRL
210	220	230	240	250
ERGQEYLILE	KNDVHWWRAR	DKYGNEGYIP	SNYVTGKKSN	NLDQYEWYCR
260	270	280	290	300
NMNRSKAEQL	LRSEDKEGGF	MVRDSSQPGL	YTVSLYTKFG	GEGSSGFRHY
310	320	330	340	350
HIKETTTSPK	KYYLAEKHAF	GSIPEIIEYH	KHNAAGLVTR	LRYPVSVKGK
360	370	380	390	400
NAPTTAGFSY	EKWEINPSEL	TFMRELGSGL	FGVVRLGKWR	AQYKVAIKAI
410	420	430	440	450
REGAMCEEDF	IEEAKVMMKL	THPKLVQLYG	VCTQQKPIYI	VTEFMERGCL
460	470	480	490	500
LNFLRQRQGH	FSRDVLLSMC	QDVCEGMEYL	ERNSFIHRDL	AARNCLVSEA
510	520	530	540	550
GVVKVSDFGM	ARYVLDDQYT	SSSGAKFPVK	WCPPEVFNYS	RFSSKSDVWS
560	570	580	590	600
FGVLMWEVFT	EGRMPFEKYT	NYEVVTMVTR	GHRLYQPKLA	SNYVYEVMLR
610	620	630	631
CWQEKPEGRP	SFEDLLRTID	ELVECEETFG	R

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-3137 Collapse assay details

Data source: Panorama

Official Gene Symbol: TEC
Peptide Sequence: HAFGSIPEIIEYHK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 318
Peptide End: 331

CPTAC ID: CPTAC-3137
Peptide Molecular Mass: 1,639.8358
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b5 (1+)	12.4	8.4	9.1	11.4	9.6	8.9	16.8	12.8	12.7	15	15	15
y5 (1+)	12	7.7	8	10.5	8.1	8.1	15.9	11.2	11.4	15	15	15
b6 (1+)	9.2	9.8	7.3	11	8.2	8.2	14.3	12.8	11	15	15	15
sum	10.8	8.5	8	10.3	8.4	8.2	14.9	12	11.5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3136 Collapse assay details

Data source: Panorama

Official Gene Symbol: TEC
Peptide Modified Sequence: NDDGVIPC[+57.0]QNK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 75
Peptide - Site of Modification: 8
Peptide Start: 67
Peptide End: 77

CPTAC ID: CPTAC-3136
Peptide Molecular Mass: 1,258.5612
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend