CSNK1D, CPTAC-3148 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CSNK1D	HCKID

Sequence Length (AA)	Molecular Weight (Da)
415	47330

Protein Name
Casein kinase I isoform delta

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MELRVGNRYR	LGRKIGSGSF	GDIYLGTDIA	AGEEVAIKLE	CVKTKHPQLH
60	70	80	90	100
IESKIYKMMQ	GGVGIPTIRW	CGAEGDYNVM	VMELLGPSLE	DLFNFCSRKF
110	120	130	140	150
SLKTVLLLAD	QMISRIEYIH	SKNFIHRDVK	PDNFLMGLGK	KGNLVYIIDF
160	170	180	190	200
GLAKKYRDAR	THQHIPYREN	KNLTGTARYA	SINTHLGIEQ	SRRDDLESLG
210	220	230	240	250
YVLMYFNLGS	LPWQGLKAAT	KRQKYERISE	KKMSTPIEVL	CKGYPSEFAT
260	270	280	290	300
YLNFCRSLRF	DDKPDYSYLR	QLFRNLFHRQ	GFSYDYVFDW	NMLKFGASRA
310	320	330	340	350
ADDAERERRD	REERLRHSRN	PATRGLPSTA	SGRLRGTQEV	APPTPLTPTS
360	370	380	390	400
HTANTSPRPV	SGMERERKVS	MRLHRGAPVN	ISSSDLTGRQ	DTSRMSTSQI
410	415
PGRVASSGLQ	SVVHR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-3149 Collapse assay details

Data source: Panorama

Official Gene Symbol: CSNK1D
Peptide Sequence: GAPVNISSSDLTGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 376
Peptide End: 389

CPTAC ID: CPTAC-3149
Peptide Molecular Mass: 1,372.6947
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	6.2	4.4	4.3	7.4	3.9	4.1	9.7	5.9	5.9	15	15	15
y8 (1+)	4.2	3.8	4.8	6	4.2	4.1	7.3	5.7	6.3	15	15	15
y10 (1+)	4.3	3.6	4.8	5.9	3.9	4.3	7.3	5.3	6.4	15	15	15
sum	4	3.7	4.5	6	3.9	4.1	7.2	5.4	6.1	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3148 Collapse assay details

Data source: Panorama

Official Gene Symbol: CSNK1D
Peptide Sequence: VASSGLQSVVHR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 404
Peptide End: 415

CPTAC ID: CPTAC-3148
Peptide Molecular Mass: 1,238.6731
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend