WEE1, CPTAC-3168 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
WEE1	N/A

Sequence Length (AA)	Molecular Weight (Da)
646	71597

Protein Name
Wee1-like protein kinase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSFLSRQQPP	PPRRAGAACT	LRQKLIFSPC	SDCEEEEEEE	EEEGSGHSTG
60	70	80	90	100
EDSAFQEPDS	PLPPARSPTE	PGPERRRSPG	PAPGSPGELE	EDLLLPGACP
110	120	130	140	150
GADEAGGGAE	GDSWEEEGFG	SSSPVKSPAA	PYFLGSSFSP	VRCGGPGDAS
160	170	180	190	200
PRGCGARRAG	EGRRSPRPDH	PGTPPHKTFR	KLRLFDTPHT	PKSLLSKARG
210	220	230	240	250
IDSSSVKLRG	SSLFMDTEKS	GKREFDVRQT	PQVNINPFTP	DSLLLHSSGQ
260	270	280	290	300
CRRRKRTYWN	DSCGEDMEAS	DYELEDETRP	AKRITITESN	MKSRYTTEFH
310	320	330	340	350
ELEKIGSGEF	GSVFKCVKRL	DGCIYAIKRS	KKPLAGSVDE	QNALREVYAH
360	370	380	390	400
AVLGQHSHVV	RYFSAWAEDD	HMLIQNEYCN	GGSLADAISE	NYRIMSYFKE
410	420	430	440	450
AELKDLLLQV	GRGLRYIHSM	SLVHMDIKPS	NIFISRTSIP	NAASEEGDED
460	470	480	490	500
DWASNKVMFK	IGDLGHVTRI	SSPQVEEGDS	RFLANEVLQE	NYTHLPKADI
510	520	530	540	550
FALALTVVCA	AGAEPLPRNG	DQWHEIRQGR	LPRIPQVLSQ	EFTELLKVMI
560	570	580	590	600
HPDPERRPSA	MALVKHSVLL	SASRKSAEQL	RIELNAEKFK	NSLLQKELKK
610	620	630	640	646
AQMAKAAAEE	RALFTDRMAT	RSTTQSNRTS	RLIGKKMNRS	VSLTIY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3169 Collapse assay details

Data source: Panorama

Official Gene Symbol: WEE1
Peptide Sequence: FLANEVLQENYTHLPK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 482
Peptide End: 497

CPTAC ID: CPTAC-3169
Peptide Molecular Mass: 1,914.9840
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	9.7	7.5	5.9	20.6	7.8	5.7	22.8	10.8	8.2	15	15	15
y3 (1+)	19.8	8.4	7.2	20.5	13.2	8.9	28.5	15.6	11.4	15	15	15
y9 (1+)	10	6.3	5.2	10.9	6.2	5.7	14.8	8.8	7.7	15	15	15
sum	9	5.9	5.5	11.6	5.6	5.5	14.7	8.1	7.8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3168 Collapse assay details

Data source: Panorama

Official Gene Symbol: WEE1
Peptide Sequence: HSVLLSASR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 566
Peptide End: 574

CPTAC ID: CPTAC-3168
Peptide Molecular Mass: 968.5403
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend