FLT1, CPTAC-3174 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
FLT1	FLT, FRT, VEGFR1

Sequence Length (AA)	Molecular Weight (Da)
1338	150769

Protein Name
Vascular endothelial growth factor receptor 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVSYWDTGVL	LCALLSCLLL	TGSSSGSKLK	DPELSLKGTQ	HIMQAGQTLH
60	70	80	90	100
LQCRGEAAHK	WSLPEMVSKE	SERLSITKSA	CGRNGKQFCS	TLTLNTAQAN
110	120	130	140	150
HTGFYSCKYL	AVPTSKKKET	ESAIYIFISD	TGRPFVEMYS	EIPEIIHMTE
160	170	180	190	200
GRELVIPCRV	TSPNITVTLK	KFPLDTLIPD	GKRIIWDSRK	GFIISNATYK
210	220	230	240	250
EIGLLTCEAT	VNGHLYKTNY	LTHRQTNTII	DVQISTPRPV	KLLRGHTLVL
260	270	280	290	300
NCTATTPLNT	RVQMTWSYPD	EKNKRASVRR	RIDQSNSHAN	IFYSVLTIDK
310	320	330	340	350
MQNKDKGLYT	CRVRSGPSFK	SVNTSVHIYD	KAFITVKHRK	QQVLETVAGK
360	370	380	390	400
RSYRLSMKVK	AFPSPEVVWL	KDGLPATEKS	ARYLTRGYSL	IIKDVTEEDA
410	420	430	440	450
GNYTILLSIK	QSNVFKNLTA	TLIVNVKPQI	YEKAVSSFPD	PALYPLGSRQ
460	470	480	490	500
ILTCTAYGIP	QPTIKWFWHP	CNHNHSEARC	DFCSNNEESF	ILDADSNMGN
510	520	530	540	550
RIESITQRMA	IIEGKNKMAS	TLVVADSRIS	GIYICIASNK	VGTVGRNISF
560	570	580	590	600
YITDVPNGFH	VNLEKMPTEG	EDLKLSCTVN	KFLYRDVTWI	LLRTVNNRTM
610	620	630	640	650
HYSISKQKMA	ITKEHSITLN	LTIMNVSLQD	SGTYACRARN	VYTGEEILQK
660	670	680	690	700
KEITIRDQEA	PYLLRNLSDH	TVAISSSTTL	DCHANGVPEP	QITWFKNNHK
710	720	730	740	750
IQQEPGIILG	PGSSTLFIER	VTEEDEGVYH	CKATNQKGSV	ESSAYLTVQG
760	770	780	790	800
TSDKSNLELI	TLTCTCVAAT	LFWLLLTLFI	RKMKRSSSEI	KTDYLSIIMD
810	820	830	840	850
PDEVPLDEQC	ERLPYDASKW	EFARERLKLG	KSLGRGAFGK	VVQASAFGIK
860	870	880	890	900
KSPTCRTVAV	KMLKEGATAS	EYKALMTELK	ILTHIGHHLN	VVNLLGACTK
910	920	930	940	950
QGGPLMVIVE	YCKYGNLSNY	LKSKRDLFFL	NKDAALHMEP	KKEKMEPGLE
960	970	980	990	1000
QGKKPRLDSV	TSSESFASSG	FQEDKSLSDV	EEEEDSDGFY	KEPITMEDLI
1010	1020	1030	1040	1050
SYSFQVARGM	EFLSSRKCIH	RDLAARNILL	SENNVVKICD	FGLARDIYKN
1060	1070	1080	1090	1100
PDYVRKGDTR	LPLKWMAPES	IFDKIYSTKS	DVWSYGVLLW	EIFSLGGSPY
1110	1120	1130	1140	1150
PGVQMDEDFC	SRLREGMRMR	APEYSTPEIY	QIMLDCWHRD	PKERPRFAEL
1160	1170	1180	1190	1200
VEKLGDLLQA	NVQQDGKDYI	PINAILTGNS	GFTYSTPAFS	EDFFKESISA
1210	1220	1230	1240	1250
PKFNSGSSDD	VRYVNAFKFM	SLERIKTFEE	LLPNATSMFD	DYQGDSSTLL
1260	1270	1280	1290	1300
ASPMLKRFTW	TDSKPKASLK	IDLRVTSKSK	ESGLSDVSRP	SFCHSSCGHV
1310	1320	1330	1338
SEGKRRFTYD	HAELERKIAC	CSPPPDYNSV	VLYSTPPI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-3174 Collapse assay details

Data source: Panorama

Official Gene Symbol: FLT1
Peptide Sequence: LGDLLQANVQQDGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 1154
Peptide End: 1167

CPTAC ID: CPTAC-3174
Peptide Molecular Mass: 1,497.7787
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	9.5	4.2	6.3	13.3	4	7.9	16.3	5.8	10.1	15	15	15
b3 (1+)	6.6	4.3	5.8	11.5	4.3	7.3	13.3	6.1	9.3	15	15	15
b4 (1+)	8.4	4.3	4.7	13	4.1	6.5	15.5	5.9	8	15	15	15
sum	7.2	4.1	5.6	12.1	4	7.2	14.1	5.7	9.1	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2819 Collapse assay details

Data source: Panorama

Official Gene Symbol: FLT1
Peptide Sequence: VTSPNITVTLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 160
Peptide End: 170

CPTAC ID: CPTAC-2819
Peptide Molecular Mass: 1,171.6812
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend