RPS6KB1, CPTAC-3184 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RPS6KB1	STK14A

Sequence Length (AA)	Molecular Weight (Da)
525	59140

Protein Name
Ribosomal protein S6 kinase beta-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRRRRRRDGF	YPAPDFRDRE	AEDMAGVFDI	DLDQPEDAGS	EDELEEGGQL
60	70	80	90	100
NESMDHGGVG	PYELGMEHCE	KFEISETSVN	RGPEKIRPEC	FELLRVLGKG
110	120	130	140	150
GYGKVFQVRK	VTGANTGKIF	AMKVLKKAMI	VRNAKDTAHT	KAERNILEEV
160	170	180	190	200
KHPFIVDLIY	AFQTGGKLYL	ILEYLSGGEL	FMQLEREGIF	MEDTACFYLA
210	220	230	240	250
EISMALGHLH	QKGIIYRDLK	PENIMLNHQG	HVKLTDFGLC	KESIHDGTVT
260	270	280	290	300
HTFCGTIEYM	APEILMRSGH	NRAVDWWSLG	ALMYDMLTGA	PPFTGENRKK
310	320	330	340	350
TIDKILKCKL	NLPPYLTQEA	RDLLKKLLKR	NAASRLGAGP	GDAGEVQAHP
360	370	380	390	400
FFRHINWEEL	LARKVEPPFK	PLLQSEEDVS	QFDSKFTRQT	PVDSPDDSTL
410	420	430	440	450
SESANQVFLG	FTYVAPSVLE	SVKEKFSFEP	KIRSPRRFIG	SPRTPVSPVK
460	470	480	490	500
FSPGDFWGRG	ASASTANPQT	PVEYPMETSG	IEQMDVTMSG	EASAPLPIRQ
510	520	525
PNSGPYKKQA	FPMISKRPEH	LRMNL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3184 Collapse assay details

Data source: Panorama

Official Gene Symbol: RPS6KB1
Peptide Modified Sequence: IRPEC[+57.0]FELLR
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 91
Peptide - Site of Modification: 5
Peptide Start: 86
Peptide End: 95

CPTAC ID: CPTAC-3184
Peptide Molecular Mass: 1,331.7020
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	5.2	3.8	4.5	13.1	3.8	4.2	14.1	5.4	6.2	15	15	15
y4 (1+)	5.6	4.5	5.3	14	4.4	4.8	15.1	6.3	7.2	15	15	15
b4 (1+)	5.4	4	4.8	14.2	4.3	4.9	15.2	5.9	6.9	15	15	15
sum	5.1	4	4.8	13.7	4	4.4	14.6	5.7	6.5	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-3183 Collapse assay details

Data source: Panorama

Official Gene Symbol: RPS6KB1
Peptide Sequence: LNLPPYLTQEAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 310
Peptide End: 321

CPTAC ID: CPTAC-3183
Peptide Molecular Mass: 1,413.7616
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend