PRKCH, CPTAC-3188 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PRKCH	PKCL, PRKCL

Sequence Length (AA)	Molecular Weight (Da)
683	77828

Protein Name
Protein kinase C eta type

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSSGTMKFNG	YLRVRIGEAV	GLQPTRWSLR	HSLFKKGHQL	LDPYLTVSVD
60	70	80	90	100
QVRVGQTSTK	QKTNKPTYNE	EFCANVTDGG	HLELAVFHET	PLGYDHFVAN
110	120	130	140	150
CTLQFQELLR	TTGASDTFEG	WVDLEPEGKV	FVVITLTGSF	TEATLQRDRI
160	170	180	190	200
FKHFTRKRQR	AMRRRVHQIN	GHKFMATYLR	QPTYCSHCRE	FIWGVFGKQG
210	220	230	240	250
YQCQVCTCVV	HKRCHHLIVT	ACTCQNNINK	VDSKIAEQRF	GINIPHKFSI
260	270	280	290	300
HNYKVPTFCD	HCGSLLWGIM	RQGLQCKICK	MNVHIRCQAN	VAPNCGVNAV
310	320	330	340	350
ELAKTLAGMG	LQPGNISPTS	KLVSRSTLRR	QGKESSKEGN	GIGVNSSNRL
360	370	380	390	400
GIDNFEFIRV	LGKGSFGKVM	LARVKETGDL	YAVKVLKKDV	ILQDDDVECT
410	420	430	440	450
MTEKRILSLA	RNHPFLTQLF	CCFQTPDRLF	FVMEFVNGGD	LMFHIQKSRR
460	470	480	490	500
FDEARARFYA	AEIISALMFL	HDKGIIYRDL	KLDNVLLDHE	GHCKLADFGM
510	520	530	540	550
CKEGICNGVT	TATFCGTPDY	IAPEILQEML	YGPAVDWWAM	GVLLYEMLCG
560	570	580	590	600
HAPFEAENED	DLFEAILNDE	VVYPTWLHED	ATGILKSFMT	KNPTMRLGSL
610	620	630	640	650
TQGGEHAILR	HPFFKEIDWA	QLNHRQIEPP	FRPRIKSRED	VSNFDPDFIK
660	670	680	683
EEPVLTPIDE	GHLPMINQDE	FRNFSYVSPE	LQP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3187 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKCH
Peptide Sequence: LGSLTQGGEHAILR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 597
Peptide End: 610

CPTAC ID: CPTAC-3187
Peptide Molecular Mass: 1,450.7892
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	6.3	2.6	5.1	6.7	3.9	5.1	9.2	4.7	7.2	15	15	15
y4 (1+)	6.9	3.4	5.8	7.9	5	5.5	10.5	6	8	15	15	15
y9 (1+)	3.2	4.7	4.4	5.9	4.9	5.7	6.7	6.8	7.2	15	15	15
sum	5.4	2.9	4.9	6.3	4	5	8.3	4.9	7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3188 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKCH
Peptide Modified Sequence: NHPFLTQLFC[+57.0]C[+57.0]FQTPDR
Modification Type: Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification: 422, 423
Peptide - Site of Modification: 10, 11
Peptide Start: 412
Peptide End: 428

CPTAC ID: CPTAC-3188
Peptide Molecular Mass: 2,179.9932
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend