IRAK3, CPTAC-3190 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
IRAK3	N/A

Sequence Length (AA)	Molecular Weight (Da)
596	67767

Protein Name
Interleukin-1 receptor-associated kinase 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGNCGARGA	LSAHTLLFDL	PPALLGELCA	VLDSCDGALG	WRGLAERLSS
60	70	80	90	100
SWLDVRHIEK	YVDQGKSGTR	ELLWSWAQKN	KTIGDLLQVL	QEMGHRRAIH
110	120	130	140	150
LITNYGAVLS	PSEKSYQEGG	FPNILFKETA	NVTVDNVLIP	EHNEKGILLK
160	170	180	190	200
SSISFQNIIE	GTRNFHKDFL	IGEGEIFEVY	RVEIQNLTYA	VKLFKQEKKM
210	220	230	240	250
QCKKHWKRFL	SELEVLLLFH	HPNILELAAY	FTETEKFCLI	YPYMRNGTLF
260	270	280	290	300
DRLQCVGDTA	PLPWHIRIGI	LIGISKAIHY	LHNVQPCSVI	CGSISSANIL
310	320	330	340	350
LDDQFQPKLT	DFAMAHFRSH	LEHQSCTINM	TSSSSKHLWY	MPEEYIRQGK
360	370	380	390	400
LSIKTDVYSF	GIVIMEVLTG	CRVVLDDPKH	IQLRDLLREL	MEKRGLDSCL
410	420	430	440	450
SFLDKKVPPC	PRNFSAKLFC	LAGRCAATRA	KLRPSMDEVL	NTLESTQASL
460	470	480	490	500
YFAEDPPTSL	KSFRCPSPLF	LENVPSIPVE	DDESQNNNLL	PSDEGLRIDR
510	520	530	540	550
MTQKTPFECS	QSEVMFLSLD	KKPESKRNEE	ACNMPSSSCE	ESWFPKYIVP
560	570	580	590	596
SQDLRPYKVN	IDPSSEAPGH	SCRSRPVESS	CSSKFSWDEY	EQYKKE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-3190 Collapse assay details

Data source: Panorama

Official Gene Symbol: IRAK3
Peptide Sequence: SSISFQNIIEGTR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 151
Peptide End: 163

CPTAC ID: CPTAC-3190
Peptide Molecular Mass: 1,450.7416
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	8.7	6	9.4	9.6	9.5	10.7	13	11.2	14.2	15	15	15
y10 (1+)	8.3	6.2	9.4	9.8	9.9	9.6	12.8	11.7	13.4	15	15	15
y7 (1+)	7.5	5.6	9.3	7.9	8.8	10.2	10.9	10.4	13.8	15	15	15
sum	8	5.8	9.3	8.9	9.4	10.1	12	11	13.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3189 Collapse assay details

Data source: Panorama

Official Gene Symbol: IRAK3
Peptide Sequence: VEIQNLTYAVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 182
Peptide End: 192

CPTAC ID: CPTAC-3189
Peptide Molecular Mass: 1,276.7027
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend