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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CDK2 CDKN2
Sequence Length (AA) Molecular Weight (Da)
298 33930
Protein Name
Cyclin-dependent kinase 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MENFQKVEKI GEGTYGVVYK ARNKLTGEVV ALKKIRLDTE TEGVPSTAIR
60 70 80 90 100
EISLLKELNH PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP
110 120 130 140 150
LPLIKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINTEGA IKLADFGLAR
160 170 180 190 200
AFGVPVRTYT HEVVTLWYRA PEILLGCKYY STAVDIWSLG CIFAEMVTRR
210 220 230 240 250
ALFPGDSEID QLFRIFRTLG TPDEVVWPGV TSMPDYKPSF PKWARQDFSK
260 270 280 290 298
VVPPLDEDGR SLLSQMLHYD PNKRISAKAA LAHPFFQDVT KPVPHLRL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3191 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK2
Peptide Modified Sequence
APEILLGC[+57.0]K
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
178
Peptide - Site of Modification
8
Peptide Start
170
Peptide End
178
CPTAC ID
CPTAC-3191
Peptide Molecular Mass
999.5423
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6 4.7 5.7 4.5 7.4 7.8 7.5 8.8 9.7 15 15 15
y3 (1+) 8.4 4.7 5.2 7.8 6.7 5.7 11.5 8.2 7.7 15 15 15
y5 (1+) 4.8 5.6 5.8 5 7.2 7.3 6.9 9.1 9.3 15 15 15
sum 5.7 4.8 5.4 5.1 6.9 6.7 7.6 8.4 8.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3193 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK2
Peptide Sequence
ELNHPNIVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
57
Peptide End
65
CPTAC ID
CPTAC-3193
Peptide Molecular Mass
1,062.5822
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 22.1 10.4 16.1 27.9 25.9 25.7 35.6 27.9 30.3 15 15 15
y5 (1+) 5.8 5.2 5.7 7.2 4.2 5.6 9.2 6.7 8 15 15 15
b7 (1+) 15.6 4.7 3.8 18.6 5.8 4.8 24.3 7.5 6.1 15 15 15
sum 7.2 5.7 6.7 9.5 6 9.2 11.9 8.3 11.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3192 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK2
Peptide Sequence
LTGEVVALK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
25
Peptide End
33
CPTAC ID
CPTAC-3192
Peptide Molecular Mass
928.5593
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 3.9 3.9 6.9 5.1 4.5 6.9 6.4 6 9.8 15 15 15
y3 (1+) 9.5 3.3 4.1 9.6 3.7 4.4 13.5 5 6 15 15 15
y4 (1+) 8 4.3 6 11.2 6.2 6.6 13.8 7.5 8.9 15 15 15
sum 4.5 2.8 5.8 5.3 3.9 5.8 7 4.8 8.2 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3194 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK2
Peptide Sequence
TYTHEVVTLWYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
158
Peptide End
169
CPTAC ID
CPTAC-3194
Peptide Molecular Mass
1,566.7831
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 9.8 9.4 16.1 13.5 20 18.2 16.7 22.1 24.3 15 15 15
y3 (1+) 11.1 6.9 6.3 11.5 12.6 10.7 16 14.4 12.4 15 15 15
y5 (1+) 9.6 7.9 12.6 10.7 16.2 15.3 14.4 18 19.8 15 15 15
sum 7.1 7.1 9.8 7.9 14.6 13.2 10.6 16.2 16.4 15 15 15


Additional Resources and Comments