DCK, CPTAC-3198 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
DCK	N/A

Sequence Length (AA)	Molecular Weight (Da)
260	30519

Protein Name
Deoxycytidine kinase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MATPPKRSCP	SFSASSEGTR	IKKISIEGNI	AAGKSTFVNI	LKQLCEDWEV
60	70	80	90	100
VPEPVARWCN	VQSTQDEFEE	LTMSQKNGGN	VLQMMYEKPE	RWSFTFQTYA
110	120	130	140	150
CLSRIRAQLA	SLNGKLKDAE	KPVLFFERSV	YSDRYIFASN	LYESECMNET
160	170	180	190	200
EWTIYQDWHD	WMNNQFGQSL	ELDGIIYLQA	TPETCLHRIY	LRGRNEEQGI
210	220	230	240	250
PLEYLEKLHY	KHESWLLHRT	LKTNFDYLQE	VPILTLDVNE	DFKDKYESLV
260
EKVKEFLSTL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3199 Collapse assay details

Data source: Panorama

Official Gene Symbol: DCK
Peptide Sequence: DAEKPVLFFER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 118
Peptide End: 128

CPTAC ID: CPTAC-3199
Peptide Molecular Mass: 1,349.6980
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b6 (1+)	5.8	3.6	3.7	10.1	4.4	5.4	11.6	5.7	6.5	15	15	15
y7 (1+)	6.8	4.9	4.2	10.1	5.7	5	12.2	7.5	6.5	15	15	15
y4 (1+)	4.7	3.9	3.3	10	4	4.7	11	5.6	5.7	15	15	15
sum	4.6	3.8	3.1	9.8	4.2	4.6	10.8	5.7	5.5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-3198 Collapse assay details

Data source: Panorama

Official Gene Symbol: DCK
Peptide Sequence: NEEQGIPLEYLEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 195
Peptide End: 207

CPTAC ID: CPTAC-3198
Peptide Molecular Mass: 1,560.7672
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend