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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

TYK2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TYK2 N/A
Sequence Length (AA) Molecular Weight (Da)
1187 133650
Protein Name
Non-receptor tyrosine-protein kinase TYK2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPLRHWGMAR GSKPVGDGAQ PMAAMGGLKV LLHWAGPGGG EPWVTFSESS
60 70 80 90 100
LTAEEVCIHI AHKVGITPPC FNLFALFDAQ AQVWLPPNHI LEIPRDASLM
110 120 130 140 150
LYFRIRFYFR NWHGMNPREP AVYRCGPPGT EASSDQTAQG MQLLDPASFE
160 170 180 190 200
YLFEQGKHEF VNDVASLWEL STEEEIHHFK NESLGMAFLH LCHLALRHGI
210 220 230 240 250
PLEEVAKKTS FKDCIPRSFR RHIRQHSALT RLRLRNVFRR FLRDFQPGRL
260 270 280 290 300
SQQMVMVKYL ATLERLAPRF GTERVPVCHL RLLAQAEGEP CYIRDSGVAP
310 320 330 340 350
TDPGPESAAG PPTHEVLVTG TGGIQWWPVE EEVNKEEGSS GSSGRNPQAS
360 370 380 390 400
LFGKKAKAHK AVGQPADRPR EPLWAYFCDF RDITHVVLKE HCVSIHRQDN
410 420 430 440 450
KCLELSLPSR AAALSFVSLV DGYFRLTADS SHYLCHEVAP PRLVMSIRDG
460 470 480 490 500
IHGPLLEPFV QAKLRPEDGL YLIHWSTSHP YRLILTVAQR SQAPDGMQSL
510 520 530 540 550
RLRKFPIEQQ DGAFVLEGWG RSFPSVRELG AALQGCLLRA GDDCFSLRRC
560 570 580 590 600
CLPQPGETSN LIIMRGARAS PRTLNLSQLS FHRVDQKEIT QLSHLGQGTR
610 620 630 640 650
TNVYEGRLRV EGSGDPEEGK MDDEDPLVPG RDRGQELRVV LKVLDPSHHD
660 670 680 690 700
IALAFYETAS LMSQVSHTHL AFVHGVCVRG PENIMVTEYV EHGPLDVWLR
710 720 730 740 750
RERGHVPMAW KMVVAQQLAS ALSYLENKNL VHGNVCGRNI LLARLGLAEG
760 770 780 790 800
TSPFIKLSDP GVGLGALSRE ERVERIPWLA PECLPGGANS LSTAMDKWGF
810 820 830 840 850
GATLLEICFD GEAPLQSRSP SEKEHFYQRQ HRLPEPSCPQ LATLTSQCLT
860 870 880 890 900
YEPTQRPSFR TILRDLTRLQ PHNLADVLTV NPDSPASDPT VFHKRYLKKI
910 920 930 940 950
RDLGEGHFGK VSLYCYDPTN DGTGEMVAVK ALKADCGPQH RSGWKQEIDI
960 970 980 990 1000
LRTLYHEHII KYKGCCEDQG EKSLQLVMEY VPLGSLRDYL PRHSIGLAQL
1010 1020 1030 1040 1050
LLFAQQICEG MAYLHAQHYI HRDLAARNVL LDNDRLVKIG DFGLAKAVPE
1060 1070 1080 1090 1100
GHEYYRVRED GDSPVFWYAP ECLKEYKFYY ASDVWSFGVT LYELLTHCDS
1110 1120 1130 1140 1150
SQSPPTKFLE LIGIAQGQMT VLRLTELLER GERLPRPDKC PCEVYHLMKN
1160 1170 1180 1187
CWETEASFRP TFENLIPILK TVHEKYQGQA PSVFSVC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3207 Collapse assay details

Data source: Panorama

Official Gene Symbol
TYK2
Peptide Modified Sequence
LTADSSHYLC[+57.0]HEVAPPR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
436
Peptide - Site of Modification
10
Peptide Start
426
Peptide End
442
CPTAC ID
CPTAC-3207
Peptide Molecular Mass
1,951.9210
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 12.2 6.2 3.2 17.1 6 3.9 21 8.6 5 15 15 15
y4 (1+) 13.5 7.2 3.5 16.8 6.9 5 21.6 10 6.1 15 15 15
y5 (1+) 10.6 7.2 3.6 14.9 7 4.4 18.3 10 5.7 15 15 15
sum 11.9 6.9 3.2 16 6.6 4.3 19.9 9.5 5.4 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for CPTAC-2821 Collapse assay details

Data source: Panorama

Official Gene Symbol
TYK2
Peptide Modified Sequence
NLVHGNVC[+57.0]GR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
736
Peptide - Site of Modification
8
Peptide Start
729
Peptide End
738
CPTAC ID
CPTAC-2821
Peptide Molecular Mass
1,124.5509
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 8.9 3.6 1.8 12.3 5.5 2.5 15.2 6.6 3.1 15 15 15
y5 (1+) 103.7 8.3 5.1 93.8 11.1 4.7 139.8 13.9 6.9 15 15 15
y4 (1+) 35.6 6.8 5.4 44.7 6.7 6.3 57.1 9.5 8.3 15 15 15
sum 9.3 3.6 2.1 12 5.4 2.9 15.2 6.5 3.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-3206 Collapse assay details

Data source: Panorama

Official Gene Symbol
TYK2
Peptide Sequence
TLNLSQLSFHR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
573
Peptide End
583
CPTAC ID
CPTAC-3206
Peptide Molecular Mass
1,314.7044
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 4.3 4.2 9.3 12.9 4.4 9.6 13.6 6.1 13.4 15 15 15
y7 (1+) 5.1 5 10 14.6 5.2 9.3 15.5 7.2 13.7 15 15 15
y4 (1+) 4.3 5.3 9 11.6 10.6 10.2 12.4 11.9 13.6 15 15 15
sum 4.2 4.4 9.1 12.9 5.6 9.3 13.6 7.1 13 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments