STK24, CPTAC-3208 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STK24	MST3, STK3

Sequence Length (AA)	Molecular Weight (Da)
443	49308

Protein Name
Serine/threonine-protein kinase 24

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDSRAQLWGL	ALNKRRATLP	HPGGSTNLKA	DPEELFTKLE	KIGKGSFGEV
60	70	80	90	100
FKGIDNRTQK	VVAIKIIDLE	EAEDEIEDIQ	QEITVLSQCD	SPYVTKYYGS
110	120	130	140	150
YLKDTKLWII	MEYLGGGSAL	DLLEPGPLDE	TQIATILREI	LKGLDYLHSE
160	170	180	190	200
KKIHRDIKAA	NVLLSEHGEV	KLADFGVAGQ	LTDTQIKRNT	FVGTPFWMAP
210	220	230	240	250
EVIKQSAYDS	KADIWSLGIT	AIELARGEPP	HSELHPMKVL	FLIPKNNPPT
260	270	280	290	300
LEGNYSKPLK	EFVEACLNKE	PSFRPTAKEL	LKHKFILRNA	KKTSYLTELI
310	320	330	340	350
DRYKRWKAEQ	SHDDSSSEDS	DAETDGQASG	GSDSGDWIFT	IREKDPKNLE
360	370	380	390	400
NGALQPSDLD	RNKMKDIPKR	PFSQCLSTII	SPLFAELKEK	SQACGGNLGS
410	420	430	440	443
IEELRGAIYL	AEEACPGISD	TMVAQLVQRL	QRYSLSGGGT	SSH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3209 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK24
Peptide Sequence: NLENGALQPSDLDR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 348
Peptide End: 361

CPTAC ID: CPTAC-3209
Peptide Molecular Mass: 1,540.7481
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	3.3	2.8	2.6	6.7	3.9	3.1	7.5	4.8	4	15	15	15
y6 (1+)	4.3	2.6	1.7	7.4	3.1	3.7	8.6	4	4.1	15	15	15
y8 (1+)	4.8	2.4	2	6.7	3	3.2	8.2	3.8	3.8	15	15	15
sum	3.7	2.5	1.7	6.9	3.1	3.3	7.8	4	3.7	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-3208 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK24
Peptide Modified Sequence: SQAC[+57.0]GGNLGSIEELR
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 395
Peptide - Site of Modification: 4
Peptide Start: 391
Peptide End: 405

CPTAC ID: CPTAC-3208
Peptide Molecular Mass: 1,589.7468
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend