BRIP1, CPTAC-3220 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
BRIP1	BACH1, FANCJ

Sequence Length (AA)	Molecular Weight (Da)
1249	140878

Protein Name
Fanconi anemia group J protein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSSMWSEYTI	GGVKIYFPYK	AYPSQLAMMN	SILRGLNSKQ	HCLLESPTGS
60	70	80	90	100
GKSLALLCSA	LAWQQSLSGK	PADEGVSEKA	EVQLSCCCAC	HSKDFTNNDM
110	120	130	140	150
NQGTSRHFNY	PSTPPSERNG	TSSTCQDSPE	KTTLAAKLSA	KKQASIYRDE
160	170	180	190	200
NDDFQVEKKR	IRPLETTQQI	RKRHCFGTEV	HNLDAKVDSG	KTVKLNSPLE
210	220	230	240	250
KINSFSPQKP	PGHCSRCCCS	TKQGNSQESS	NTIKKDHTGK	SKIPKIYFGT
260	270	280	290	300
RTHKQIAQIT	RELRRTAYSG	VPMTILSSRD	HTCVHPEVVG	NFNRNEKCME
310	320	330	340	350
LLDGKNGKSC	YFYHGVHKIS	DQHTLQTFQG	MCKAWDIEEL	VSLGKKLKAC
360	370	380	390	400
PYYTARELIQ	DADIIFCPYN	YLLDAQIRES	MDLNLKEQVV	ILDEAHNIED
410	420	430	440	450
CARESASYSV	TEVQLRFARD	ELDSMVNNNI	RKKDHEPLRA	VCCSLINWLE
460	470	480	490	500
ANAEYLVERD	YESACKIWSG	NEMLLTLHKM	GITTATFPIL	QGHFSAVLQK
510	520	530	540	550
EEKISPIYGK	EEAREVPVIS	ASTQIMLKGL	FMVLDYLFRQ	NSRFADDYKI
560	570	580	590	600
AIQQTYSWTN	QIDISDKNGL	LVLPKNKKRS	RQKTAVHVLN	FWCLNPAVAF
610	620	630	640	650
SDINGKVQTI	VLTSGTLSPM	KSFSSELGVT	FTIQLEANHI	IKNSQVWVGT
660	670	680	690	700
IGSGPKGRNL	CATFQNTETF	EFQDEVGALL	LSVCQTVSQG	ILCFLPSYKL
710	720	730	740	750
LEKLKERWLS	TGLWHNLELV	KTVIVEPQGG	EKTNFDELLQ	VYYDAIKYKG
760	770	780	790	800
EKDGALLVAV	CRGKVSEGLD	FSDDNARAVI	TIGIPFPNVK	DLQVELKRQY
810	820	830	840	850
NDHHSKLRGL	LPGRQWYEIQ	AYRALNQALG	RCIRHRNDWG	ALILVDDRFR
860	870	880	890	900
NNPSRYISGL	SKWVRQQIQH	HSTFESALES	LAEFSKKHQK	VLNVSIKDRT
910	920	930	940	950
NIQDNESTLE	VTSLKYSTPP	YLLEAASHLS	PENFVEDEAK	ICVQELQCPK
960	970	980	990	1000
IITKNSPLPS	SIISRKEKND	PVFLEEAGKA	EKIVISRSTS	PTFNKQTKRV
1010	1020	1030	1040	1050
SWSSFNSLGQ	YFTGKIPKAT	PELGSSENSA	SSPPRFKTEK	MESKTVLPFT
1060	1070	1080	1090	1100
DKCESSNLTV	NTSFGSCPQS	ETIISSLKID	ATLTRKNHSE	HPLCSEEALD
1110	1120	1130	1140	1150
PDIELSLVSE	EDKQSTSNRD	FETEAEDESI	YFTPELYDPE	DTDEEKNDLA
1160	1170	1180	1190	1200
ETDRGNRLAN	NSDCILAKDL	FEIRTIKEVD	SAREVKAEDC	IDTKLNGILH
1210	1220	1230	1240	1249
IEESKIDDID	GNVKTTWINE	LELGKTHEIE	IKNFKPSPSK	NKGMFPGFK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-918 Collapse assay details

Data source: Panorama

Official Gene Symbol: BRIP1
Peptide Modified Sequence: ATPELGSSENSASS[+80.0]PPR
Modification Type: Phospho (ST)
Protein - Site of Modification: 1032
Peptide - Site of Modification: 14
Peptide Start: 1019
Peptide End: 1035

CPTAC ID: CPTAC-918
Peptide Molecular Mass: 1,765.7520
Species: Homo sapiens (Human)
Assay Type: Enrichment ImmunoMRM
Enrichment Method: peptide immunoaffinity
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: Peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y15 (2+)	21.5	9	8.8	24	10.4	10.9	32.2	13.8	14	15	24	15
y10 (1+)	38.4	29.3	22.3	50.1	30.2	26.1	63.1	42.1	34.3	15	24	15
y11 (1+)	33.9	16.2	15.8	39.1	21.6	22.5	51.7	27	27.5	15	24	15
sum	20.2	8.2	8.5	24.1	10	10.5	31.4	12.9	13.5	15	24	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	42.7	23.6	14.2	39.5	30.8	16.4	58.2	38.8	21.7	15	15	15
y9 (1+)	27.6	20.6	12.8	30	22.4	13.1	40.8	30.4	18.3	15	15	15
y10 (1+)	38.8	20.2	9.5	53	17.6	12.1	65.7	26.8	15.4	15	15	15
y11 (1+)	33.5	14.9	9.8	33.7	20.7	12.4	47.5	25.5	15.8	15	15	15
sum	17.7	10.5	6.7	16.2	13.4	6.4	24	17	9.3	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y15 (2+)	23.4	14.2	8.3	37.9	12	9.3	44.5	18.6	12.5	15	15	15
y3 (1+)	43.2	8.3	13.1	50	9.2	15.6	66.1	12.4	20.4	15	15	15
y11 (1+)	40.3	47.2	15.5	45.2	56.1	16.7	60.6	73.3	22.8	15	15	15
sum	19.8	10.6	7.7	35.5	9.6	9.1	40.6	14.3	11.9	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y18 (2+)	27.5	18.5	22.3	32.5	27.8	27.4	42.6	33.4	35.3	15	15	15
y21 (2+)	16.5	10.6	14.5	26.9	18.3	23.2	31.6	21.1	27.4	15	15	15
y22 (2+)	10.5	8.7	7.3	15.6	20.9	16.9	18.8	22.6	18.4	15	15	15
y10 (1+)	26.6	15.2	21.6	27.7	18.5	33.4	38.4	23.9	39.8	15	15	15
sum	9	7.7	6.2	15.3	18.7	17.6	17.8	20.2	18.7	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (2+)	28.1	37.7	45.3	44.2	42.5	126.7	52.4	56.8	134.6	15	15	15
y19 (2+)	39.4	41.7	67.1	36.5	55.4	92.4	53.7	69.3	114.2	15	15	15
y21 (2+)	26.9	22.4	37.5	40.9	31.5	46.2	49	38.7	59.5	15	15	15
y10 (1+)	45.1	42.3	41.7	47.6	51.6	49.6	65.6	66.7	64.8	15	15	15
sum	18.9	19.4	20.2	21.3	30.5	36.1	28.5	36.1	41.4	15	15	15

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BRIP1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-918 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

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Assay Details for CPTAC-3221 Collapse assay details

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Assay Details for CPTAC-3222 Collapse assay details

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Assay Details for CPTAC-3220 Collapse assay details

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Assay Details for CPTAC-3281 Collapse assay details

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