TERF2, CPTAC-3256 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TERF2	TRBF2, TRF2

Sequence Length (AA)	Molecular Weight (Da)
542	59594

Protein Name
Telomeric repeat-binding factor 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAGAGTAGP	ASGPGVVRDP	AASQPRKRPG	REGGEGARRS	DTMAGGGGSS
60	70	80	90	100
DGSGRAAGRR	ASRSSGRARR	GRHEPGLGGP	AERGAGEARL	EEAVNRWVLK
110	120	130	140	150
FYFHEALRAF	RGSRYGDFRQ	IRDIMQALLV	RPLGKEHTVS	RLLRVMQCLS
160	170	180	190	200
RIEEGENLDC	SFDMEAELTP	LESAINVLEM	IKTEFTLTEA	VVESSRKLVK
210	220	230	240	250
EAAVIICIKN	KEFEKASKIL	KKHMSKDPTT	QKLRNDLLNI	IREKNLAHPV
260	270	280	290	300
IQNFSYETFQ	QKMLRFLESH	LDDAEPYLLT	MAKKALKSES	AASSTGKEDK
310	320	330	340	350
QPAPGPVEKP	PREPARQLRN	PPTTIGMMTL	KAAFKTLSGA	QDSEAAFAKL
360	370	380	390	400
DQKDLVLPTQ	ALPASPALKN	KRPRKDENES	SAPADGEGGS	ELQPKNKRMT
410	420	430	440	450
ISRLVLEEDS	QSTEPSAGLN	SSQEAASAPP	SKPTVLNQPL	PGEKNPKVPK
460	470	480	490	500
GKWNSSNGVE	EKETWVEEDE	LFQVQAAPDE	DSTTNITKKQ	KWTVEESEWV
510	520	530	540	542
KAGVQKYGEG	NWAAISKNYP	FVNRTAVMIK	DRWRTMKRLG	MN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-956 Collapse assay details

Data source: Panorama

Official Gene Symbol: TERF2
Peptide Modified Sequence: DLVLPTQALPAS[+80.0]PALK
Modification Type: Phospho (ST)
Protein - Site of Modification: 365
Peptide - Site of Modification: 12
Peptide Start: 354
Peptide End: 369

CPTAC ID: CPTAC-956
Peptide Molecular Mass: 1,712.9114
Species: Homo sapiens (Human)
Assay Type: Enrichment ImmunoMRM
Enrichment Method: peptide immunoaffinity
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: Peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (2+)	46.6	25.3	22.6	87.2	32.4	30.8	98.9	41.1	38.2	15	24	15
y13 (2+)	46.7	29.7	18.4	69.8	33.7	18.4	84	44.9	26	15	24	15
y7 (1+)	44.4	16	14	66.5	25	15.7	80	29.7	21	15	24	15
sum	30	12.6	9.7	65.4	22.9	14.3	72	26.1	17.3	15	24	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments